摘要
目的为溃疡宁胶囊质量控制提供新方法。方法色谱柱：Agilent hypersil ODS（4．6mm×250mm，5μm）；流动相：甲醇-水（70：30）；检测波长：280hm；柱温：35℃；流速：1．0mL·min^-1。结果靛蓝线性范围：0．07763～0．5802μg·mL^-1。相关系数r分别为0．9998。回收率为100．1％，RSD为1．24％。结论本方法简便、可靠、重现性好，能起到控制溃疡宁胶囊中靛蓝含量的作用。
OBJECTIVE To develop an effective quality control method for Kuiyangning capsules. METHODS Indigotin in Kulyangning capsules was determined by RP-HPLC, using Agilent hypersil ODS （ 4. 6 ×250mm, 5 μm） column, methanol-water （ 70： 30） as mobile phase and UV detector was set at 280 nm. The flow rate was 1, 0 mL·min^-1. RESULTS The linearity range of in- digotin was 0.07763 -0. 5802μg ·mL^-1, the average recovery was 100.1% with RSD 1.24%. CONCLUSION The method is simple, rapid, accurate and repreatable. It can be used for determination of indigotin in Kuiyangning capsules.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2007年第1期74-77,共4页
Chinese Journal of Modern Applied Pharmacy
