Two new glutarimide derivatives,2-hydroxy-3-[2-(2-hydroxy-3-methylphenyl-5-hydroxymethyl)-2-oxoethyl]glutarimide(1)and 2-hydroxy-3-[2-[2-hydroxy-3-hydroxymethyl-5-methylphenyl]-2-oxoethyl]glutarimide(2),along with 8 k...Two new glutarimide derivatives,2-hydroxy-3-[2-(2-hydroxy-3-methylphenyl-5-hydroxymethyl)-2-oxoethyl]glutarimide(1)and 2-hydroxy-3-[2-[2-hydroxy-3-hydroxymethyl-5-methylphenyl]-2-oxoethyl]glutarimide(2),along with 8 known compounds were isolated from the liquid fermentation broth of endophytic Streptomyces sp.YINM00048 obtained from Agrimonia pilosa.Their structures were elucidated through comprehensive spectroscopic analysis,including nuclear magnetic resonance,high-resolution mass spectrometry,and single-crystal X-ray diffraction,supported by time-dependent density functional theory(TDDFT)-electronic circular dichroism(ECD)calculations.Furthermore,compound 2 displayed weak cytotoxic activity against A549 and SMMC-7721 cell lines with IC50 values of 62.69 and 55.07μmol/L,respectively.展开更多
To get the IR spectrums of Agrimonia Pilosa Ledeb （APL） from China and Japan areas, and to fend out the characters of IR spectrums through the content of different chemical constituents, to provide a fast and effect...To get the IR spectrums of Agrimonia Pilosa Ledeb （APL） from China and Japan areas, and to fend out the characters of IR spectrums through the content of different chemical constituents, to provide a fast and effective analysis method monitor the inherent qualities of traditional Chinese medicine-APL. Fourier Transform Infrared Spectroscopy （FT-IR） was applied to detect sample of APL from Ofina and Japan areas. This study showed that the IR spectra of APL from China and Japan areas have their unique IR fingerprint features. The contents of tannin and calcitum phosphate in APL from China is different APL from Japan. So FF-IR is a very quick, effective and well repetitive method for monitoring and distinguishing the traditional Chinese medicine.展开更多
[ Objectives] This study was conducted to compare agrimophol contentsin Agrimonia pilosa Ledeb. at different harvest time, so as to select its best harvest time. [Methods] High performance liquid chromatography was us...[ Objectives] This study was conducted to compare agrimophol contentsin Agrimonia pilosa Ledeb. at different harvest time, so as to select its best harvest time. [Methods] High performance liquid chromatography was used for the determination of agfmophol in A. pilosa Ledeb. at different harvest time in Luzhou. [ Results ] The content of agrimophol could be detected by HPLC. Agrimophol had good linearity in 0.01 - 0.1 mg/ml （ r = 0.999 9 ）, the average recov- ery was 101.5%, and RSD was 1.10%. The content of agrimophol in A. pilosa Ledeb. in Luzhou was 0.010 8% in June, and 0.013 1% in July, much higher than any other months. [ Conclusions] This experiment established a method for detecting the content of agrimophol by HPLC, which is simple, with high precision, high accuracy, good repeatability and recovery. The reasonable harvest time of A. pilosa Ledeb. in Luzhou is June and July.展开更多
The aim of the study is the development and standardization of granules in hard gelatin capsules based on the composition of dry extracts of Bidens tripartita L.,Solidago canadensis L.and Agrimonia eupatoria L.Herbs,p...The aim of the study is the development and standardization of granules in hard gelatin capsules based on the composition of dry extracts of Bidens tripartita L.,Solidago canadensis L.and Agrimonia eupatoria L.Herbs,possessing nephroprotective activity and antifibrotics in toxic nephropathies.Developed the structure and defined the quality indicators of the pellets with dry extracts of Bidens tripartita L.,Solidago canadensis L.and Agrimonia eupatoria L.herbs.The quality specification for the drug-granules of the composition of dry extracts in solid gelatin capsules is developed.展开更多
The accumulation of senescent cells induces age-related dysfunction in various organs,and the selective clearance of senescent cells is a potential approach for treating age-related diseases.Hence,identifying natural ...The accumulation of senescent cells induces age-related dysfunction in various organs,and the selective clearance of senescent cells is a potential approach for treating age-related diseases.Hence,identifying natural foods that provide senolytic benefits is important because they may serve as a preventative measure and an alternative to pharmaceutical interventions for aging that mitigates the potential side effects associated with drug-based approaches.In this study,we investigated the senolytic activity of ethanol extracts derived from Agrimonia pilosa Ledeb(APL-E)and agrimol B,an active component of the plant,in doxorubicin-induced senescent WI38 cells.APL-E and agrimol B decreased senescence-associated-β-galactosidase(SA-β-gal)-positive senescent WI38 cells in a concentration-dependent manner.Annexin V staining revealed that APL-E and agrimol B caused apoptosis in senescent cells.To investigate the effect of APL-E on senolytic activity in vivo,aged male C57BL/6J mice were fed APL-E for 3 months.SA-β-gal staining revealed significantly decreased senescent cells in the liver and kidney of APL-E-fed aged mice.The expression levels of senescence-associated secretory phenotype factors Il1β,Ccl2,and Cxcl9 were also decreased in the livers and kidneys of APE-fed mice.The number of senescent CD8^(+)T cells in the blood decreased in APL-E-fed aged mice.Furthermore,APL-E-fed mice showed improvement in aging-induced immune senescence,marked by increased naïve CD8^(+)T cells and decreased memory CD8^(+)T cells.This study provides evidence of the novel senolytic activity of APL-E and agrimol B and suggests their application as preventatives against various age-related disorders.展开更多
文摘Two new glutarimide derivatives,2-hydroxy-3-[2-(2-hydroxy-3-methylphenyl-5-hydroxymethyl)-2-oxoethyl]glutarimide(1)and 2-hydroxy-3-[2-[2-hydroxy-3-hydroxymethyl-5-methylphenyl]-2-oxoethyl]glutarimide(2),along with 8 known compounds were isolated from the liquid fermentation broth of endophytic Streptomyces sp.YINM00048 obtained from Agrimonia pilosa.Their structures were elucidated through comprehensive spectroscopic analysis,including nuclear magnetic resonance,high-resolution mass spectrometry,and single-crystal X-ray diffraction,supported by time-dependent density functional theory(TDDFT)-electronic circular dichroism(ECD)calculations.Furthermore,compound 2 displayed weak cytotoxic activity against A549 and SMMC-7721 cell lines with IC50 values of 62.69 and 55.07μmol/L,respectively.
基金supported by Research Foundation for Science and Technology Innovation Talents of Harbin(2010RFQXS072)Scientific Research Foundation for the Returned Overseas Chinese Scholars of Heilongjiang Province of China(200923)
文摘To get the IR spectrums of Agrimonia Pilosa Ledeb （APL） from China and Japan areas, and to fend out the characters of IR spectrums through the content of different chemical constituents, to provide a fast and effective analysis method monitor the inherent qualities of traditional Chinese medicine-APL. Fourier Transform Infrared Spectroscopy （FT-IR） was applied to detect sample of APL from Ofina and Japan areas. This study showed that the IR spectra of APL from China and Japan areas have their unique IR fingerprint features. The contents of tannin and calcitum phosphate in APL from China is different APL from Japan. So FF-IR is a very quick, effective and well repetitive method for monitoring and distinguishing the traditional Chinese medicine.
文摘[ Objectives] This study was conducted to compare agrimophol contentsin Agrimonia pilosa Ledeb. at different harvest time, so as to select its best harvest time. [Methods] High performance liquid chromatography was used for the determination of agfmophol in A. pilosa Ledeb. at different harvest time in Luzhou. [ Results ] The content of agrimophol could be detected by HPLC. Agrimophol had good linearity in 0.01 - 0.1 mg/ml （ r = 0.999 9 ）, the average recov- ery was 101.5%, and RSD was 1.10%. The content of agrimophol in A. pilosa Ledeb. in Luzhou was 0.010 8% in June, and 0.013 1% in July, much higher than any other months. [ Conclusions] This experiment established a method for detecting the content of agrimophol by HPLC, which is simple, with high precision, high accuracy, good repeatability and recovery. The reasonable harvest time of A. pilosa Ledeb. in Luzhou is June and July.
文摘The aim of the study is the development and standardization of granules in hard gelatin capsules based on the composition of dry extracts of Bidens tripartita L.,Solidago canadensis L.and Agrimonia eupatoria L.Herbs,possessing nephroprotective activity and antifibrotics in toxic nephropathies.Developed the structure and defined the quality indicators of the pellets with dry extracts of Bidens tripartita L.,Solidago canadensis L.and Agrimonia eupatoria L.herbs.The quality specification for the drug-granules of the composition of dry extracts in solid gelatin capsules is developed.
文摘The accumulation of senescent cells induces age-related dysfunction in various organs,and the selective clearance of senescent cells is a potential approach for treating age-related diseases.Hence,identifying natural foods that provide senolytic benefits is important because they may serve as a preventative measure and an alternative to pharmaceutical interventions for aging that mitigates the potential side effects associated with drug-based approaches.In this study,we investigated the senolytic activity of ethanol extracts derived from Agrimonia pilosa Ledeb(APL-E)and agrimol B,an active component of the plant,in doxorubicin-induced senescent WI38 cells.APL-E and agrimol B decreased senescence-associated-β-galactosidase(SA-β-gal)-positive senescent WI38 cells in a concentration-dependent manner.Annexin V staining revealed that APL-E and agrimol B caused apoptosis in senescent cells.To investigate the effect of APL-E on senolytic activity in vivo,aged male C57BL/6J mice were fed APL-E for 3 months.SA-β-gal staining revealed significantly decreased senescent cells in the liver and kidney of APL-E-fed aged mice.The expression levels of senescence-associated secretory phenotype factors Il1β,Ccl2,and Cxcl9 were also decreased in the livers and kidneys of APE-fed mice.The number of senescent CD8^(+)T cells in the blood decreased in APL-E-fed aged mice.Furthermore,APL-E-fed mice showed improvement in aging-induced immune senescence,marked by increased naïve CD8^(+)T cells and decreased memory CD8^(+)T cells.This study provides evidence of the novel senolytic activity of APL-E and agrimol B and suggests their application as preventatives against various age-related disorders.
