摘要
为了提高龙牙草(Agrimonia pilosa Ledeb.)多酚的提取率,根据单因素试验结果,选择液料比(A)、超声时间(B)、SHMP添加量(C)、超声功率(D)作为影响因素,以多酚得率为响应值,设计4因素3水平响应面试验对多酚提取工艺进行优化,并测定多酚的抗氧化能力。结果表明,龙牙草多酚提取的最佳工艺条件为液料比25∶1(mL/g)、超声时间40 min、SHMP添加量0.5%、超声功率160 W,该条件下的多酚得率为4.35%。龙牙草多酚浓度在0.1~1.5 mg/mL时,其对超氧阴离子自由基、DPPH自由基、羟基自由基的最大清除率分别为59.80%、43.61%、32.72%,且清除能力随多酚浓度的增加而变强,证明龙牙草多酚具有抗氧化能力。
To improve the extraction rate of polyphenols from Agrimonia pilosa Ledeb.,liquid-to-material ratio(A),ultrasonic time(B),SHMP addition(C),and ultrasonic power(D)were selected as influencing factors based on single-factor experiment results.A four-factor three-level response surface methodology was designed with polyphenol yield as the response value to optimize the extraction process,and the antioxidant capacity of the polyphenols was determined.The results showed that the optimal extraction conditions for polyphenols from Agrimonia pilosa Ledeb.were the liquid-to-material ratio of 25∶1(mL/g),ultrasonic time of 40 min,SHMP addition of 0.5%,and ultrasonic power of 160 W,with a polyphenol yield of 4.35%under these conditions.When the concentration of polyphenols from Agrimonia pilosa Ledeb.ranged from 0.1 to 1.5 mg/mL,the maximum scavenging rates for superoxide anion radicals,DPPH radicals,and hydroxyl radicals were 59.80%,43.61%,and 32.72%,respectively.The scavenging capacity increased with polyphenol concentration,demonstrating the antioxidant ability of Agrimonia pilosa Ledeb.polyphenols.
作者
李明珠
袁磊
张学真
颜晓倩
LI Ming-zhu;YUAN Lei;ZHANG Xue-zhen;YAN Xiao-qian(School of Culinary Arts,Qingdao Preschool Education College,Qingdao 266300,Shandong,China;School of Food Engineering,Qingdao Institute of Technology,Qingdao 266300,Shandong,China)
出处
《湖北农业科学》
2025年第5期128-133,共6页
Hubei Agricultural Sciences
基金
山东省高等学校基础科学(自然科学)研究项目(24GJZ10022)。
