摘要
为探究复方仙鹤草肠炎胶囊(compound Agrimonia pilosula enteritis capsules,CAPEC)治疗溃疡性结肠炎(ulcerative colitis,UC)湿热证的效应机制,将小鼠随机分为5组:对照组、模型组、阳性药5-氨基水杨酸(5-aminosalicylic acid,5-ASA)组、CAPEC低剂量(CAPEC-L)组、CAPEC高剂量(CAPEC-H)组,采用高糖高脂饮食、白酒饲喂联合葡聚糖硫酸钠建立UC湿热证小鼠模型。通过酶联免疫吸附实验、实时荧光定量PCR、三重四极杆液质联用系统、组织病理学分析等方法,研究了CAPEC对UC湿热证小鼠模型胆汁中胆汁酸(bile acids,BAs)代谢谱及FXR-SREBP-1信号通路的影响。实验结果显示,与模型组相比,CAPEC-L组及CAPEC-H组小鼠疾病活动指数、血清及结肠组织中IL-6、IL-1β、TNF-α等促炎因子水平均显著降低;肠道炎症、肝脏脂质蓄积及舌组织异常改变明显改善。CAPEC-H组胆汁中BAs谱的异常增高显著降低;肝脏Cyp7a1、Cyp7b1、Cyp27a1、Bsep、Fxr、Shp mRNA水平上调;肝脏Srebp-1、Cyp8b1 mRNA水平下调。本研究证实了CAPEC对UC湿热证的改善作用,并提示该作用与其调控胆汁酸代谢、改善肝脏脂质蓄积和肠道炎症有关,为揭示CAPEC清热燥湿、健脾止泻的效应机制提供了线索。
This study aimed to investigate the mechanism of compound Agrimonia pilosula enteritis capsules(CAPEC)on ulcerative colitis(UC)in mice with dampness-heat syndrome.The mice were randomly divided into five groups:the control group,the model group,the positive drug(5-aminosalicylic acid,5-ASA)group,the lowdose CAPEC(CAPEC-L)group and the high-dose CAPEC(CAPEC-H)group.The mice models were established by using high-fat high-sucrose diet,feeding with distilled spirit and dextran sulfate sodium(DSS).The effects of CAPEC on bile acids(BAs)metabolic profiles in bile and the FXR-SREBP-1 signaling pathway were investigated in the model of UC in mice with dampness-heat syndrome by ELISA,qRT-PCR,UHPLCQQQ/MS,and histopathological analysis.The results showed that,compared with the model group,the CAPEC-L group and the CAPEC-H group significantly reduced the disease activity index(DAI),and proinflammatory cytokine levels(including IL-6,IL-1β,and TNF-α)in both serum and colon tissues.Additionally,CAPEC markedly ameliorated intestinal inflammation,hepatic lipid accumulation,and pathological alterations in tongue tissue.The CAPEC-H group significantly attenuated the abnormal elevation of BAs profiles in bile,and upregulated hepatic mRNA levels of Cyp7a1,Cyp7b1,Cyp27a1,Bsep,Fxr,and Shp,while down-regulating Srebp-1 and Cyp8b1 expression.The experimental results suggest that CAPEC alleviates UC with dampness-heat syndrome by ameliorating BAs metabolic disorders,hepatic lipid accumulation,and intestinal inflammation.These findings provide mechanistic insights into CAPEC’s traditional effects of clearing heat and drying dampness,and strengthening the spleen to relieve diarrhea.
作者
姚深梦
章真
闻晓东
王霞
YAO Shenmeng;ZHANG Zhen;WEN Xiaodong;WANG Xia(School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 211198;Nanjing Pukou Hospital of Traditional Chinese Medicine,the Affiliated Hospital of China Pharmaceutical University,Nanjing 211800,China)
出处
《中国药科大学学报》
北大核心
2026年第1期78-89,共12页
Journal of China Pharmaceutical University
基金
国家自然科学基金项目(No.82374024)
江苏省中医药科技发展计划项目(MS2024054)。
关键词
复方仙鹤草肠炎胶囊
湿热证
溃疡性结肠炎
胆汁酸代谢
compound Agrimonia pilosula enteritis capsule
dampness-heat syndrome
ulcerative colitis
bile acid metabolism
