摘要
目的 了解肝癌患者乙型肝炎病毒 (HBV)DNA含量及其与C基因启动子 (BCP)基因变异的关系。方法 采用PCR荧光实时定量技术检测 1 1 4例肝癌患者及 1 0 0例非肝癌乙肝患者HBV DNA含量。采用PCR 微板核酸分子杂交ELISA技术对肝癌及乙肝患者进行BCP基因变异检测。结果 肝癌患者 4 8%HBV DNA阳性 ,平均拷贝量为 4 .7× 1 0 6拷贝 /ml。BCP区域 176 2、1 76 4位突变率为 2 7% ,且BCP变异的患者HBV拷贝量明显大于非变异患者。 1 0 0例非肝癌乙肝患者HBV DNA阳性率4 1 % ,平均拷贝量 3.8× 1 0 5拷贝 /ml,BCP基因突变率为 8% ,肝癌患者的BCP基因突变率明显高于乙肝患者。结论 HBV感染可能是导致肝癌发生的重要原因 ,HBVBCP变异可能与病变程度有关。
Objective To investigate the relationship between HBV DNA concentrations and BCP gene mutation of hepatoma patients.Methods FQ PCR method was applied to detect HBV DNA concentration in 114 hepatomas and 100 B type hepatitis patients,PCR microplate nucletide aid hybridization ELISA technique was applied in the analysis of BCP gene mutation.Results The HBV DNA positive rate of hepatoma patients was 48%,the average HBV DNA concentration was 4.7×10 6copies/ml.The BCP gene mutation rate was 27%,HBV DNA concentration of BCP mutation samples wase significantly higher than that no mutation samples.The HBV DNA positive rate of B type hepatitis patients was 41%,the average HBV DNA concentration was 3.8×10 5copies/ml.The BCP gene mutation rate was 8%.The BCP gene mutation rate of hepatoma remarkably higher than of B type hepatitis patients.Conclusions HBV infection is the important reason of leading to hepatoma,HBV BCP mutation is related to symptoms degree.
出处
《重庆医学》
CAS
CSCD
2004年第6期880-881,共2页
Chongqing medicine
