摘要
为探索ＨＢＶ 前Ｃ区Ａ８３ 点突变与临床关系，采用错配ＰＣＲ（ｍｐ － ＰＣＲ） 与限制性长度多态性分析（ＲＦＬＰ） 相结合的方法，对６６ 例乙肝患者的血清标本，进行了ＨＢＶ前Ｃ区Ａ８３ 点突变的检测，结果：６６ 例患者中，３６ 例（５４－５ ％ ） 检出了Ａ８３ 点突变。慢肝中度、重度、肝炎肝硬变组明显高于ＨＢＶ 携带者（ Ｐ＜ ０－０５） ，抗－ ＨＢｅ 阳性组的检出率为７３－２ ％ ，明显高于ＨＢｅＡｇ 阳性组，而这些病例中，９１－６ ％ 发生了慢性活动性肝病，提示前Ｃ区Ａ８３ 点突变的发生，可能与机体长期的炎症活动、免疫压力的选择有关。
To investigate the relationship between the HBV preC gene mutation and clinic,we detected the variation of A83 at HBV preC gene by the methods of mp PCR and RFLP in sera of 66 patients.The results showed that thirty of them (54 5%) had the preC A83 mutation and prevalence of the mutation of asymptomatic HBV carriers(ASC),mild chronic hepatitis B(CHB),moderate CHB,severe CHB and cirrhosis after hepatitis B was 12 5%,42 9%,65 4%,69 2% and 60 0% respectively.The rates of the preC A83 mutation in moderate CHB and severe CHB and cirrhosis after hepatitis B were higher than that in ASC(P<0 05)and they were also higher in patients with anti HBe positive than in patients with anti HBe negative.91 6% of the anti HBe positive patients were belong to chronic active liver diseases.The result sugges that the preC A83 mutation might be related to the long time inflammatory action of liver and to the immune pressure in infected hosts.
出处
《临床肝胆病杂志》
CAS
北大核心
1999年第4期218-219,224,共3页
Journal of Clinical Hepatology
关键词
HBV
乙型肝炎
突变
病理
Hepatitis B Virus Hepatitis B Mutation
