摘要
我们前已报道用抗ＨＢＶｘ抗原（ＨＢｘＡｇ）三种不同肽段的抗体检出肝癌细胞中ＨＢｘＡｇ的存在。我们用ＰＣＲ技术扩增肝癌肝组织中的ｘ基因，用重组ＨＢｘＡｇ及其抗体分别检测肝癌患者血清中的抗ＨＢｘ和ＨＢｘＡｇ并用免疫组织化学的方法分析肝组织中ＨＢｘＡｇ的表达及其分布。我们发现ＨＢｘＡｇ在肝癌肝组织中阳性率高达９２．６％而且分布广泛，主要表达在细胞浆，但细胞核内亦有见到。癌旁组织检出率也很高，而且在小胆管上皮细胞内亦见有ＨＢｘＡｇ的存在。
In our
previous papers intrahepatic localization of HB xAg in liver cancer
was investigated usingantibodies against peptide fragments
representing three particular regions of X gene sequence. In
thisstudy PCR technique was used to detect the X gene in the liver
tissue sections.The antisera against therecombinant HBxAg which was
synthesized in E. coli were prepared andused for the detection
ofHBxAg in the liver cell of 7 8 patients with HCC. And the
HBxAg/anti HBx were also analizedin the sera from the patients using
ELISA. It was found that HBxAg was not only seen in hepatocyte
cyto-plasm but also in nuclei and the total positivity rate of HBxAg
is 92.6%(75/78). Besides this, we alsofound the predominant
expression of HBXAg in bile duct ethelial cells. Our findings show
that theexpression of HBxAg closely related to the development of
HCC.
关键词
肝细胞癌
乙型肝炎病毒
X抗原
基因表达
Hepatocellular carcinoma Hepatitis B virus x antigen
Gene expression
