摘要
作者曾报道过日本佩兰植物中存在生物碱,本文进一步证明提取的生物碱可能是由几种不同生物碱共同组成。该生物总碱在体外试验中表现出一定的抗肿瘤活性,在103.4±9.8μg/ml的浓度下,对体外培养的HeLa细胞有50％的抑制率。体内试验表明腹腔注射日本佩兰生物总碱50mg/kg·d连续7天,腹水型S180肉瘤小鼠的生存期限显著延长,二次实验生命延长率分别为33.93％及44.43％。单用环磷酰胺15mg/kg·d连续7天,小鼠生命延长率为56.59％及61.43％。若与生物总碱合用,生命延长率可提高至84.40％及94.64％。皮下注射生物总碱时小鼠生命延长率不明显,但与环磷酰胺合用,其生命延长率亦可从51.33％及57.24％提高至78.53％及87.93％。结果表明腹腔注射或皮下注射生物总碱与环磷酰胺合用时,均呈现协同作用。
The extraction and identification of the mixed alkaloid fromEupato-rium japonicum Thumb plant was reported previously. The more purified total alkaloid included many alkaloids which were detected by thin-layer chromatography.In vitro, the 50%inhibition(IC50) of HeLa cell growth was 103.4±9.8ug/ mg alkaloid, which indicated that the alkaloid possessed cytotoxicity. In vivo, the increase life sp-ans(ILS) of S180 ascitic tumor bearing mice in two experiments were as follows: total alkaloid 50mg/ kg/ d×7(ip.) induced inhibition rate of 33.93%and 44.43%, GTX 15mg/ kg/ dx7(sc) -56.59%and 61.43%, but the ILS increased to 84.4%and 94.64%after the combination of these 2 drugs. When the alkaloid was injected subcutaneously, the Life span was not significantly Loner as compared to the control group. However, the ILS increased from51.33% and 57.24% to 78.53%and 87. 93%, when the alkaloid (sc) was combined with CTX. These results suggested that the alkaloid showed synergismwith CTX.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1993年第3期203-206,共4页
Chinese Journal of Cancer
关键词
日本佩兰
生物碱
抗癌作用
Eupatorhom japonicum thunb Alkaloid Anitumor activity Synergism
