摘要
目的:观察辟秽防感香囊预防流感的临床疗效及其对免疫功能的调节作用。方法:临床观察部分将幼儿园239例儿童按班级随机分为用药组(118例)和空白对照组(121例),用药组儿童佩戴辟秽防感香囊,连续观察45d,观察流感的患病率、病程,并记录其临床症状。动物实验中将40只昆明种小鼠随机分为4组:正常对照组、免疫低下模型组、模型用药组和正常用药组,环磷酰胺腹腔注射制造小鼠免疫低下模型,造模后模型用药组和正常用药组给予辟秽防感香囊提取液滴鼻,连续用药1周后处死动物,检测其脾指数、T细胞亚群计数、血清γ干扰素(interfero nγ,IFN-γ)含量、自然杀伤细胞(natural killer,NK)杀伤活性和呼吸道分泌物中分泌型免疫球蛋白A(secretory immunoglobulin A,SIgA)含量。结果:用药组儿童的流感患病率和平均病程均明显低于空白对照组(P<0.01),发热、鼻塞流涕、咽部充血等症状和体征也较对照组有所缓解(P<0.05,P<0.01);动物腹腔给予环磷酰胺后,其脾指数、CD3+和CD4+细胞数、CD4+/CD8+比值、NK细胞杀伤活性、血清INF-γ水平及呼吸道SIgA含量均显著低于正常对照组(P<0.05或P<0.01),提示处于免疫低下状态,当给予辟秽防感香囊提取液滴鼻1周后,模型动物的脾指数和呼吸道SIgA含量明显升高,CD3+和CD4+细胞数、CD4+/CD8+比值及血清INF-γ呈现上升趋势,但药物对正常动物的免疫功能影响不大。结论:辟秽防感香囊对流行性感冒和普通感冒有较好的预防作用,能提高免疫功能低下小鼠的免疫水平,尤其是提高呼吸道黏膜免疫功能。
Objective：To study the clinical efficacy of Pihui Fanggan Sachet （PHFGS）,a sachet of traditional Chinese herbs,in preventing influenza and its immune regulation on mice.Methods：In clinical study,239 children from Shanghai Baoshan Xubeihong Art Kindergarten were randomly divided into two groups according to different class; 118 children were treated with PHFGS for 45 days as treatment group and 121 children were as blank control.During the observation period,the incidence rate of influenza,the course of disease and the severity of symptoms were recorded.In experimental study,40 Kunming mice were randomly divided into normal control group （normal mice treated or not treated with PHFGS）,and immunocompromised group （immunocompromised mice treated or not treated with PHFGS）.Immunocompromise was induced by intraperitoneal injection of 80 mg/kg cyclophosphamide for 3 days.Some mice in the normal control and immunocompromised groups were then treated with extracted solution of PHFGS through nasal cavity for one week.Spleen index,content of CD3＋ T cells and CD4＋ T cells,CD4＋/CD8＋ratio,activity of natural killer （NK） cells,serum level of interferon γ （INF-γ） and respiratory level of secretory immunoglobulin A （SIgA） were evaluated.Results：The incidence rate of influenza in the treatment group was much lower than that in the blank control group （P0.01）,and the average course was shortened as compared with the control group （P0.01）.The fever,rhinocleisis,runny nose,and throat congestion in the treatment group were improved as compared with the blank control group （P0.05,P0.01）.After injection of cyclophosphamide,the spleen index,content of CD3＋T cells and CD4＋T cells,CD4＋/CD8＋ ratio,activity of NK cells,serum level of INF-γ and respiratory level of SIgA in the immunocompromised group were significantly lower than those in the normal control group （P0.05 or P0.01）,which indicated the immunosuppression.After treated with PHFGS for one week,the spleen index and the respiratory level of SIgA in the immunocompromised group were improved significantly.Although the content of CD3＋ T cells and CD4＋ T cells,CD4＋/CD8＋ ratio and the serum level of INF-γ were improved,the differences did not reach the significant level.No significant effects on immune function of normal mice were observed.Conclusion：PHFGS can prevent influenza effectively by improving the immunity,especially the respiratory mucosal immune function.
出处
《中西医结合学报》
CAS
2010年第10期949-954,共6页
Journal of Chinese Integrative Medicine
基金
上海市卫生局中医药科研基金资助项目(No.2008J001A)
第二军医大学灾害医学研究专项课题资助项目
关键词
流感
预防
防御机制
患病率
免疫标记物
小鼠
influenza
prevention
defense mechanisms
prevalence
immunologic markers
mice
