摘要
目的 研究灯盏花素对大鼠局灶性脑缺血再灌注后脑水肿和中性粒细胞浸润的影响 ,探讨其抑制脑缺血再灌注损伤后炎症反应的作用机制。方法大鼠大脑中动脉短暂阻塞制成局灶性脑缺血 2h ,再灌注 2 4h模型。再灌注后 1h分别ip灯盏花素 5 0或 75mg·kg- 1,再灌注后 2 2h重复给药 1次。再灌注 2 4h后干湿重法测定脑水肿 ,分光光度法测定缺血区大脑皮质和尾壳核髓过氧化物酶 (MPO)活性 ,免疫组织化学染色测定大脑皮质和尾壳核细胞间粘附分子 1(ICAM 1)的表达。结果 缺血再灌注后 ,脑组织含水量明显增加 ,缺血区大脑皮质及尾壳核MPO活性和ICAM 1表示显著增加 ,灯盏花素 5 0和75mg·kg- 1治疗用药能减轻脑水肿 ,降低MPO活性和抑制ICAM 1表达。
AIM To investigate whether breviscapine attenuates brain edema and neutrophil infiltration and inhibiton ICAM 1 expression in the cerebral ischemia reperfusion rats. METHODS Focal cerebral ischemia reperfusion model in rats were made by transient occlusion of the middle cerebral artery for 2 h followed by 24 h reperfusion. Breviscapine 50 and 75 mg·kg-1 were administered ip at 1 h and repeated at 22 h after reperfusion. Twenty four hours later, brain edema, neutrophil infiltration and the expression of intercellular adhesion molecule 1(ICAM 1) were measured with dry wet weight, myeloperoxidase(MPO) activity, and immunohistochemistry, respectively. RESULTS After ischemia reperfusion, brain water content was obviously increased, MPO activity and the expression of ICAM 1 in the ischemic hemisphere cortex of MCA area and caudate putamen were markedly increased. Treatment with breviscapine 50 and 75 mg·kg-1 reduced brain edema, inhibited MPO activity and the expression of ICAM 1. CONCLUSION Breviscapine attenuated brain edema and neutrophil infiltration after cerebral ischemia reperfusion.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
2004年第3期161-165,共5页
Chinese Journal of Pharmacology and Toxicology
关键词
灯盏花素
脑缺血
脑水肿
中性粒细胞
细胞间粘附分子-1
breviscapine
cerebral ischemia
brain edema
neutrophils
intercellular adhesion molecule-1
