摘要
目的探讨重型颅脑损伤(traumatic brain injury,TBI)及应用甘露醇后脑组织含水量及水通道蛋白-4(AQP4)表达变化,及其与脑损伤后脑水肿之间的关系。方法将24只小鼠随机分为3组,应用自由落体打击法,制作重度颅脑损伤模型(TBI组)、假手术组(SO组)及TBI加注甘露醇(实验组)。每组于伤后8h取出小鼠损伤脑组织采用干湿重法、免疫组化方法分别计算脑组织含水量及AQP4的变化。结果损伤后8h实验组脑组织含水量较TBI组降低,但仍高于SO组。实验组AQP4表达较TBI组降低,但也高于SO组。结论AQP4与损伤后脑水肿的形成有密切关系,在探索治疗脑水肿的新型脱水药中发挥着重要作用。
Objective To investigate the effect of Mannitol on brain water content and AQP4 expression in severe traumatic brain injury （TBI）and the relationship between the brain edema and AQP4 expression. Methods Twenty-four mice were randomly divided into three groups：TBI group,sham operation group and TBI ＋ Mannitol group（experimental group）. The brains of the animals were taken out 8h after TBI. The AQP4 expression was detected by using immunohistochemistry. The wet-dry weighing method was used to detect the brain water content. Results The brain water content of the experimental group 8 h after TBI was lower than that of TBI group,but higher than that of the sham operation group. The AQP4 expression of the experimental group was lower than that of the TBI group, but higher than that of the sham operation group. Conclusion AQP4 may have close relationship in the formation and development of brain edema, and play an important role in the exploration of new dehydration drug in the treatment of brain edema.
出处
《中国现代医生》
2009年第35期32-33,41,共3页
China Modern Doctor
