摘要
目的 研究玻璃体腔内注射基质金属蛋白酶 - 3(MMP- 3)对兔眼玻璃体视网膜界面粘附作用及玻璃体凝胶状态的影响。 方法 取健康成年灰兔 2 4只 ,随机分成实验 A、B、C组和对照组 ,每组各 6只兔 (12只眼 )。实验 A、B、C组分别注射含 5、10、2 0 ng的 MMP- 3溶液 0 .1ml,对照组注射等量的平衡盐溶液 ,于注射前后行视网膜电图 (ERG)、荧光素眼底血管造影 (FFA)及光学显微镜、透射电子显微镜检查。 结果 实验 B组于注射后 1周第 1次发现临床可见的玻璃体后脱离 (PVD)及局限性玻璃体液化 ;实验 A、B组于注射后 15周分别有 1、3只眼发现临床可见的 PVD,但玻璃体液化进展缓慢。组织病理学检查发现 ,实验A、B组于注射后 6 0 min分别有 1、3只眼形成部分性 PVD;注射后 1周全部兔眼形成部分性 PVD;注射后 15周分别有 1、3只眼形成完全性 PVD,但其空隙比较狭窄。实验 C组于注射早期可见视网膜内层炎性细胞浸润及视网膜结构的破坏 ,FFA结果显示后期有荧光素渗漏。对照组有 1只眼于注射后 15周可见局限的PVD。 结论 MMP- 3能在短时间内造成玻璃体视网膜间的分离且具有较弱的促玻璃体液化作用 ;10 ng的 MMP- 3玻璃体腔内注射对兔眼是一个安全有效的剂量 ,有可能作为玻璃体手术的辅助手段。
Objective To investigate the effects of intravitreal injection of matrix metalloproteinase-3 (MMP-3) on the vitreoretinal adhesion and the vitreous gelatin. Methods Twenty-four pigmented rabbits were randomly divided into 3 experimental groups (group A, B, and C)and one control group with 6 rabbits (12 eyes) in each. Different concentrations of 0.1 ml MMP-3 (5,10, 20 ng in group A, B, and C, respectively) and equivalent dose of balanced salt solution were intravitreally injected to the rabbits, respectively. Clinical examinations (such as gross observation, slit-lamp biomicroscopy, indirect fundus ophthalmoscopy ), electroretinography (ERG) and fundus fluorecein angiography (FFA) were taken before and after injection. Results One week after injection, posterior vitreous detachment (PVD) and focal vitreous liquefaction were recognized clinically for the first time in 1 eye in group B. By the end of this study, clinically detected PVD developed in 1 eye in group A, 3 eyes in group B, but the synchisis developed slowly, and no liquefaction or PVD occurred in control group. As for the histological examination, partial PVD was observed in 1 eye in group A and 3 eyes in group B 60 minutes after injection. All of the eyes in group A and B showed partial PVD 1 week after injection, and the area of PVD enlarged in contrast with before. Complete PVD were recognized in 1 eye in group A and 3 eyes in group B 15 weeks after injection, and the cleavage was narrow and limited. In group C, inflammatory cell infiltration in the inner layer of retina, destruction of retinal structure, and fluorescein leakage at late phase was found in the early period after injection. Conclusions MMP-3 is effective in disrupting the adhesion of the posterior hyaloid to the inner limiting membrane leading to PVD, and helpful to some extent in producing vitreous liquefaction. The dose of 10 ng MMP-3 is safe and effective for the rabbits eyes, which may be used as an promising assistant of vitreous surgery.
出处
《中华眼底病杂志》
CAS
CSCD
2004年第2期111-114,共4页
Chinese Journal of Ocular Fundus Diseases
