摘要
目的:研究尿激酶联合透明质酸酶玻璃体腔内注射诱导兔眼玻璃体后脱离(PVD)的效果,并评价其安全性。方法:20只兔40眼随机分为A,B,C3组,分别为6,6,8只,均以右眼为实验眼,左眼对照。3组动物实验眼玻璃体腔内注射药物分别为:尿激酶1000U,透明质酸酶20U,尿激酶1000U+透明质酸酶20U,对照眼内均注入BBS0.1mL。术后7d内行裂隙灯、检眼镜、光镜及扫描电镜等检查。结果:术后7dA组部分性PVD发生率100%,B组为16.7%,两组均无完全性PVD发生;C组完全性PVD发生率100%,对照眼无PVD。病理学检查未发现视网膜毒性。结论:尿激酶1000U联合透明质酸酶20U兔眼玻璃体腔内注射能诱导完全性PVD,且无眼内毒性。
AIM： To investigate the effect and sefety of urokinase combined with hyaluronidase in inducing posterior vitreous detachment （PVD） in rabbits.
METHODS： 20 rabbits were devided into three groups randomly （6 in group A, 6 in group B, 8 in group C ）. All the right eyes were injected proteases and the left eyes were used as controls. Group A received a vitreous injection of Urokinase 1 000 U. Group B received hyaluronidase 20U. Group C received urokinase 1 000U and hyaluronidase 20U. All the left eyes received 0.1mL BBS. Experimental animals were observed with Slit-lamp, ophthalmoscope, and sacrificed 7d after the injections. Eyes enucleated were examined with light microscope and scanning electron microscopy.
RESULTS： Group A had the partly PVD incidence of 100%, and B 16.7%. There were no complete PVD in group A and B. The incidence of complete PVD in group C was 100%, and light microscopy confirmed the retina was intact.
CONCLUSION： Vitreous injection of urokinase 1 000U combined with hyaluronidase 20U can induce complete PVD without intraocular toxicity at 7 days alter the injection. Ukokinase or hyaluronidase alone can＇t induce complete PVD.
出处
《国际眼科杂志》
CAS
2005年第6期1164-1167,共4页
International Eye Science
关键词
尿激酶
透明质酸酶
玻璃体后脱离
兔
urokinase
hyaluronidase
posterior vitreous detachment
rabbits
