摘要
目的:研究益气养阴活血利水之复明片对兔实验性视网膜脱离后视网膜组织中MMP-2表达的影响。方法:将36只有色家兔随机分为正常对照组(A组)、模型组(B组)、西药对照组(C组)、复明片组(D组),每组9只。B、C、D组制作视网膜脱离模型。造模后第1d下午开始给药,A组与B组均予温开水灌胃,5mL/kg体重,1次/d;C组予西药混合溶液灌胃,5mL/kg体重,1次/d;D组予复明片混悬液灌胃,5mL/kg体重,1次/d。并于造模后第7、14、21天采用S-P免疫组化法检测视网膜组织中基质金属蛋白酶-2(Matrix Metallo-protein-ase-2,MMP-2)的表达。结果:MMP-2在模型组和西药对照组的RPE细胞及巨噬细胞中强阳性表达,着染深或较深;在复明片组的RPE细胞及巨噬细胞中弱阳性表达,着染浅。结论:益气养阴活血利水之复明片能下调视网膜组织中MMP-2的表达。
Objective：To investigate the effects of FuMing Tablets（FMT）of qi-benefitting,yin-fostering,and blood-invigorating and diuresis-activating on the expression of MMP-2 in the retinal tissue after detachment of the retina of rabbit.Methods： 36 rabbits were divided into normal contrast group（group A）,retinal detachment model group（group B）,western medicine contrast group（group C）,FMT group（group D）.Each group had 9 rabbits.Group B,C,D were made retinal detachment model.After models were made one day they were given medicine.Group A and B were administrated intragastrically with 5ml/kg warm water,once a day.Group C was administrated intragastrically with 5ml/kg western medicine solution,once a day.Group D was administrated intragastrically with 5ml/kg FMT suspension,once a day.Then adopting the method s-p to investigate the expression of Matrix Metallo-proteinase-2,MMP-2 in the retinal tissue respectively in the 7th day,14th day and 21st day.Results： MMP-2 takes on strong positive expression in the RPE cells of model group and west medicine group,dyeing dark or pretty dark color;while weak masculine expression in the RPE cells of FMP group,dyeing shallow color.Conclusions： FMT with the functions of benefiting qi,nourishing yin,activating blood and draining water,can decrease the expression of MMP-2 in the retinal tissue.
出处
《中华中医药学刊》
CAS
2011年第3期493-497,共5页
Chinese Archives of Traditional Chinese Medicine
基金
国家中医药管理局中医眼科学重点学科建设项目
湖南省自然科学基金资助项目(06JJ50056)
湖南省教育厅科研基金资助课题(98B078
06A052)
湖南省中医五官科学重点学科建设项目
关键词
复明片
益气养阴活血利水法
视网膜脱离模型
基质金属蛋白酶-2
兔
FuMing Tablets
method of qi-benefitting
yin-fostering
and blood-invigorating and diuresis-activating
retinal detachment model
MMP-2
rabbit.
