摘要
目的 探讨自体颗粒细胞线粒体移植 (线粒体移植 )对改善胚胎发育质量的影响。方法 对 3次行体外受精 胚胎移植 (IVF ET)治疗未获妊娠或年龄大于 37岁的 18例患者 ,采用长周期超促排卵方案促排卵。当患者取出的成熟卵子多于 4个时 ,采用常规方法 ,进行卵母细胞浆内单精子注射 (ICSI) ;或进行线粒体移植。线粒体移植的方法为收集卵丘复合体上和卵泡液中的颗粒细胞 ,经匀浆离心提取线粒体 ,应用显微操作技术将线粒体和精子一并注入卵母细胞浆内。观察、比较两种方法获得卵子的受精和胚胎发育情况。 结果 18例行超促排卵方案后 ,获得成熟卵子 16 8个 ,其中行线粒体移植卵子 86个 ,受精 6 4个 ,受精率为 74 4 % ;行ICSI卵子 82个 ,受精 6 3个 ,受精率为 76 8%。两种方法比较 ,差异无显著意义 (P >0 0 5 ) ;行线粒体移植的良好胚胎形成率为 5 9 4% ,行ICSI的良好胚胎形成率为 34 9% ,两种方法比较 ,差异有显著意义 (P <0 0 5 )。 18例中 7例临床妊娠 ,妊娠率为 38 9%。 结论 自体颗粒细胞线粒体移植可明显改善胚胎发育质量 ,提高妊娠率。
Objective To improve embryos′ quality and pregnancy rate with the method of self-mitochondria transfer. Methods All the 18 cases of women with repeated in vitro fertilization-embryo transfer (IVF-ET)-treatment failure or older than 37 years were treated with the long gonadotropin releasing hormone-agonist(GnRH-a) regimens. The oocytes were divided into two groups: intracytoplasmic sperm injection (ICSI) group and mitochondria transfer group if the number of the individual patients′ oocytes was more than four. In mitochondria transfer group, mitochondria were prepared by different centrifugation after the granulosa cells were trimmed from the oocytes or collected from follicular fluid and homogenated. Mitochondria and immobilized sperm were injected into oocytes by micromanipulation. The fertilization rate and embryos′ quality were compared. Results The fertilization rate was 74.4% in mitochondria transfer group, and 76.8 % in ICSI group,with no statistical difference( P >0.05). The good quality embryo rate in mitochondria transfer group was 59.4 %, and it was 34.9 % in ICSI group. The difference was significant ( P <0.05). There were 7 clinicl pregnancies in the 18 cases. Conclusion Self-mitochondria transfer can improve embryos′ quality and pregnancy rate without influence on fertilization rate.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2004年第2期105-107,共3页
Chinese Journal of Obstetrics and Gynecology
