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人卵母细胞形态学指标预测受精和胚胎发育潜能 被引量：4

Application of human oocyte morphometric parameters in assessment of fertilization and embryo development
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摘要 目的:探讨卵母细胞浆内单精子注射(intracytoplasmic sperm injection,ICSI)周期中人卵母细胞形态学测量的指标与受精和胚胎发育潜能之间的关系。方法:利用OCTAX Eye-ware software系统在对卵母细胞行ICSI前,逐一采集卵母细胞图像,测量透明带厚度、卵母细胞大小、极体位置的卵间隙大小和极体形态(完整或破碎),将ICSI后的卵母细胞和受精后的胚胎进行单孔培养,记录每个卵母细胞的受精情况、胚胎发育情况。对卵母细胞的形态数据与受精情况、胚胎发育情况进行逐步多元因素Logistic回归分析。结果:共测量卵母细胞436枚,受精率84.9%(370/436),胚胎可用率60.8%(225/370),卵母细胞形态学指标中,透明带厚度、极体位置的卵间隙大小和卵母细胞受精与否有关,受精卵较未受精卵有较厚的透明带[(18.0±2.3)μm vs.(16.9±2.7)μm]和较大的卵间隙[(14.4±3.3)μm vs.(13.2±3.9)μm];而卵母细胞的大小和极体形态均与取卵后72 h的胚胎发育质量有关,发育为可用胚胎的卵母细胞较发育为废用胚胎者有较大的直径[(116.6±3.7)μm vs.(114.7±3.6)μm]和较高的极体完整率(86.2%vs.66.7%)。结论:人卵母细胞形态学测量的指标可预示卵子受精和胚胎发育的潜能。 To investigate the correlation of human oocyte morphometric parameters with fertilization and embryo development in the intracytoplasmic sperm injection （ICSI） cycles. Methods： The morphometric parameters of oocytes collected and submitted to evaluation using OCTAX Eye-ware software just before ICSI. Oocyte diameter （OD）, perivitelline space width （PSW）, zonapellucida thickness （ZPT） and the shape of first polar body （FPB） （intact or fragmented） were analyzed. A stepwise multivariate Logistic regression was used to test the association between the morphometric parameters and fertilization and embryo development. Results： In the study, 436 oocytes were measured and 370 were ferti-lized （84.9%）, 225 fertilized oocytes were developed to high-quality embryos （60.8%）. ZPT and PSW were associated with fertilization. The oocytes fertilized had thicker ZPT [（18.0±2.3） μm vs. （16.9±2.7） μm] and wider PSW [（14.4±3.3） μm vs. （13.2±3.9） μm]. The OD and shape of FPB were associated with embryos development. The oocytes developed to high-quality embryos had larger OD [（116.6±3.7） μm vs. （114.7±3.6） μm] and more intact FPB （86.2% vs. 66.7%）. Conclusion： The morphometric parameters of oocytes can indicate fertilization and embryo development.
作者 孙贻娟 谷瑞环 陆小溦 赵甡 冯云
机构地区 上海交通大学医学院附属瑞金医院生殖医学中心
出处 《北京大学学报（医学版）》 CAS CSCD 北大核心 2013年第6期848-851,共4页 Journal of Peking University:Health Sciences
基金 上海市自然科学基金(09ZR1419000) 上海市科学技术委员会基础研究重点项目(10JC1410800)资助~~
关键词 卵母细胞 受精 体外 胚胎发育 Oocytes Fertilization in vitro Embryonic development
分类号 R321-33 [医药卫生—人体解剖和组织胚胎学]
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参考文献11

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同被引文献30

  • 1梁琳,陈秀娟,赵晓曦,王丽岩.卵母细胞形态及核质成熟度与体外受精结局相关性的研究进展[J].中华临床医师杂志（电子版）,2011,5(12):3567-3571. 被引量：4
  • 2刘浩,金志魁,耿春惠,帅祖兵,邱文英,付学文.卵巢刺激周期促性腺激素用量对卵母细胞形态及临床结局的影响[J].现代妇产科进展,2006,15(4):295-298. 被引量：6
  • 3Tan JH, Wang HL, Sun XS, Liu Y, Sui HS, Zhang J. Chromatin configurations in the germinal vesicle of mammalian oocytes. Mol Hum Reprod 2009; 15(1): 1-9.
  • 4LiuY, Sui HS, Wang HL, Yuan JH, Luo MJ, Xia P, et al. Germinal vesicle chromatin configurations of bovine oocytes. Microsc Res Tech 2006; 69(10): 799-807.
  • 5Liu Y, Ding B, Yartg XX, Shang MB, Lei XH, Wang R, et al. Dynamic transformation of DNA methylation and chromatin configuration in porcine oocyte during follicular growth. J Anim Vet Adv 2012; 11(10): 1739-44.
  • 6Margueron R, Reinberg D. The Polycomb complex PRC2 and its mark in life. Nature 2011; 469(7330): 343-9.
  • 7Liu Y, Ding B, Wu FR, Wang R, Yang XX, Shang MB, et al. A novel oocyte chromatin configuration classification method: based on the degree of aggregation and spatial distribution of germinal vesicle chromatin. J Anita Vet Adv 2012; 11(4): 531-8.
  • 8Bao S, Obata Y, Carroll J, Domeki I, Kono T. Epigenetic modifi- cations necessary for normal development are established during oocyte growth in mice. Biol Reprod 2000; 62(3): 616-21.
  • 9Cavilla JL, Kenedy CR, Byskov AG, Hartshome GM. Human immature oocytes grow during culture for IVM. Hum Reprod 2008; 23(1): 37-45.
  • 10Ronffto GS, Arafijo MC, de Melo AS, de Albuquerque Salles Navarro PA, Ferriani RA, Dos Reis RM. Oocyte diameter as a predictor of fertilization and embryo quality in assisted reproduction cycles. Fertil Steri12010; 93(2): 621-5.

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北京大学学报（医学版）
2013年 第6期

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