摘要
目的 探讨生存素 (survivin)蛋白在非小细胞肺癌 (NSCLC)中的表达及与细胞凋亡的关系。方法 采用免疫组化的方法检测 60例NSCLC和 16例肺良性组织中survivin的表达 ,用TUNEL技术检测 60例NSCLC的凋亡情况。结果 肺癌组织中survivin表达的阳性率为 63 .3 % ( 3 8/ 60 ) ,而在正常组织中无一例表达 ;survivin蛋白的表达与肺癌TNM分期 (P <0 .0 5 )、淋巴结转移 (P <0 .0 5 )和细胞分化程度 (P <0 .0 1)有密切关系 ,但与肿瘤组织学类型、年龄、性别无明显关系 (P >0 .0 5 )。survivin在NSCLC中的表达水平与癌细胞的凋亡指数 (AI)呈负相关 (r =-0 .2 3 1,P <0 .0 5 )。结论 survivin蛋白的异常表达而引起的细胞凋亡抑制在NSCLC的发生中起一定作用 ,其过度表达提示NSCLC预后不良 ;survivin基因有望成为肺癌基因治疗的新靶点。
Objective To investigate the expression of survivin and its relation with apoptosis in non-small cell lung cancer. Methods Survivin expression was examined in sixty paraffin-embedded NSCLC samples and 16 benign pulmonary disease tissues by immunohistochemistry methods.Apoptosis of NSCLC cells was detected by TUNEL technique. Results Survivin was expressed in 38 of 60 (63.3%) NSCLC tissues.In contrast, benign pulmonary disease tissues did not express survivin. The expression of survivin protein was closely related to TNM stages (P<0.05), lymph node involvement (P<0.05) and cell differentiation (P< 0.01), but not to histological classification, gender and ages (P>0.05). A correlation coefficient test showed a negative correlation between the AI and survivin expression (r=-0.231,P<0.05). Conclusion Apoptosis inhibition caused by abnormal survivin expression may participate in the oncogenesis and progression of NSCLC. The over-expression of survivin suggest the bad prognosis in NSCLC. Survivin gene may be indentified as a potential therapeutic target in NSCLC.
出处
《中国肺癌杂志》
CAS
2004年第2期138-141,共4页
Chinese Journal of Lung Cancer
