摘要
背景与目的survivin是新近发现的凋亡抑制因子,在肿瘤的发生中起重要作用。survivin可直接抑制caspase3和caspase7的活性。本研究旨在探讨survivin和caspase3在非小细胞肺癌(NSCLC)中的表达情况及其与肿瘤细胞凋亡的关系。方法采用免疫组织化学SP法检测88例NSCLC患者手术标本和29例正常肺组织标本中survivin和caspase3的表达,分析其与NSCLC临床病理参数之间的关系,同时用原位末端标记法(TUNEL)标记肺癌组织中的凋亡细胞。结果88例NSCLC患者标本中survivin的阳性表达率为61.4%(54/88),明显高于正常肺组织(13.8%,4/29)(P<0.01);survivin在NSCLC中的表达与肿瘤的组织学分型、病理分级和淋巴结转移均无明显相关性(P>0.05),但与TNM分期有相关性(P<0.05)。caspase3在正常肺组织和NSCLC中阳性表达率分别为89.7%(26/29)和73.9%(65/88),但二者差异无显著性(P>0.05);caspase3在NSCLC中的阳性表达率随肿瘤的病理分级增高而降低,差异有显著性(P<0.05)。survivin蛋白阳性表达的肺癌组织中平均细胞凋亡指数(1.63±0.58)明显低于阴性表达组(3.29±0.76)(P<0.05),caspase3阳性组平均细胞凋亡指数(2.42±0.59)明显高于阴性组(1.28±0.65)(P<0.05);survivin和caspase3呈负相关(P<0.05)。结论凋亡抑制蛋白survivin在NSCLC组织中高表达,caspase3与survivin的表达呈负相关,survivin可能通过抑制caspase3的激活来发挥其抑制细胞凋亡的作用。
Background and objective Survivin, a member of inhibitor of apoptosis protein （IAP） family, can directly inhibit caspase-3 and caspase-7 activity and plays an important role in oncogenesis. The aim of this study is to investigate the expressions of survivin and caspase-3 in human non-small cell lung cancer （NSCLC）, and to evaluate their relationship with cell apoptosis. Methods The expressions of survivin and caspase-3 in 88 patients with NSCLC were examined by using immunohistochemical SP methods, and TNUEL method was used to detect the cell apoptosis simultaneously. Results The positive expression rates of survivin in NSCLC tissues and normal lung tissues were 61.4% （54/88） and 13.8% （4/29） respectively, there was a significant difference between them （P〈0.01）. Survivin expression in NSCLC was not related to the histologic type, pathological grade and lymph node metastasis （P〉0. 05）, but correlated with TNM stage （P〈 0.05）. The positive expression rate of caspase-3 was 89.7% （26/29） in normal lung tissues, which was higher than that in NSCLC tissues （73.9%, 65/88）, but there was no significant difference （P〉0.05）. Caspase-3 expression in NSCLC was associated with pathological grade （P〈0.05）. The average apoptosis index （AI） of survivin-positive cases was significantly higher than that of surviving-negative ones （1.63±0.58 vs 3.29±0.76）（P〈0. 05）. The average Al of the caspase-3 positive cases was significantly higher than that of the caspase -3 negative cases（2.42±0. 59 vs 1.28±0.65）（P〈0.05）.Expression of survivin was negatively correlated with caspase-3（P〈0.05）,Conclusion Survivin may play an important role in the process of carcinogenesis of NSCLC by inbibiting cell apoptosis,Morcover,survivin may prevent cell apoptosis by inhibiting caspase-3 activation.
出处
《中国肺癌杂志》
CAS
2005年第5期435-439,共5页
Chinese Journal of Lung Cancer
基金
南京军区南京总医院科研基金(No.2003018)资助~~
