摘要
目的 研究异种 (猪 )角膜基质的生物相容性 ,评价其作为角膜体外重建载体的可行性。方法 将新鲜、脱水两种猪角膜基质分别植入新西兰白兔角膜层间 ,定期临床观察植片愈合情况 ,并于术后 2周、1、2、4、8个月取兔角膜进行组织学观察。结果 临床观察 :全部植片存活 ,12只术眼未见角膜水肿混浊、角膜新生血管、排斥反应发生 ,新鲜植片在 2个月左右已透明 ,脱水植片在 6个月后透明。组织学观察 :新鲜植片 4个月时与兔角膜基质相融愈合 ,脱水植片经角膜细胞再分布、胶原纤维改建重塑 ,于 8个月后与兔角膜基质相融愈合 ,2组植片愈合过程中未见有淋巴细胞浸润及新生血管生成。结论 异种 (猪 )角膜基质具有良好的生物相容性 ,是一种理想的角膜体外重建载体材料。
ObjectiveTo study the biocompatibility of xenogeneic swine corneal stroma as biological carrier for cornea reconstruction.MethodsThe fresh and dehydrated grafts from swine corneal stroma were implanted intralamellarly in corneas of New Zealand rabbits.Clinical examinations were performed monthly and histological examinations were taken at the 14 th,30 th,60 th,120 th and 240 th day. ResultsAll the 12 grafts were survived without rejection reaction,corneal haze or corneal neovascularization.The fresh grafts became transparent after 2 months,and the dehydrated grafts after 6 months.Histological study demonstrated that both fresh and dehydrated stroma grafts fused very well with rabbits’ corneal stroma without lymphocyte infiltrating.ConclusionSwine corneal stroma has acceptable biocompatibility,and it is an ideal biological carrier for cornea reconstruction in vitro.
出处
《眼科研究》
CAS
CSCD
北大核心
2004年第2期113-116,共4页
Chinese Ophthalmic Research
基金
国家自然科学基金 (39870 855)
广东省重点科研基金(2KM0 52 0 3S)资助
关键词
角膜基质
生物相容性
角膜重建
生物载体
异种移植
corneal stroma
biocompatibility
cornea reconstruction
biological carrier
xenograft
