摘要
目的比较脱细胞冻干羊膜与脱细胞冻干牛角膜基质在修复兔角膜浅层缺损后的机体反应,以寻找适合的组织工程角膜支架。方法新鲜健康人胎盘羊膜按罗静聪等制备方法处理,冻干后消毒备用。牛角膜经0.25%胰蛋白酶消化,生理盐水漂洗后,冻干,消毒备用。雌性日本大耳白兔20只,随机分为A、B组,每组10只。常规麻醉消毒后,采用7.5mm直径环钻造出约1/3角膜厚度的缺损。A组采用复水后的脱细胞冻干羊膜修复;B组采用脱细胞冻干牛角膜基质修复。术后裂隙灯显微镜下观察植片和新生血管状况,并于术后2、4、8周取标本,HE染色,组织学观察。结果术后两组植片无溶解、脱落,于8周降解完全,角膜中央缺损区域大部分恢复透明。两组均有新生血管长入角膜,两组新生血管长入范围比较,差异无统计学意义(P>0.05)。组织学观察2周时两组均有较重的炎性反应,成纤维细胞增生,胶原排列紊乱;4周时炎性反应均减轻,植片区域可以见到新生血管长入;8周时均有较多的成纤维细胞存在,并有少量瘢痕形成。结论脱细胞冻干羊膜与脱细胞冻干牛角膜基质修复角膜浅层缺损,仍能引起一定程度的排斥反应,需要进一步的改进。
Objective To compare the effects of the denuded freeze dried-amniotic-membrane and the denuded freeze-dried bovine corneal stroma when they were explanted to repair the corneal defect of rabbits. Methods The amnia from healthy human placentae were prepared with the method reported by LUO Jingcong, which were freeze- dried and sterilized. The bovine cornea was also denuded by typsin, rinsed, freeze-dried, and sterilized. Twenty Japan rabbits were divided into group A（the amniontic group） and group B（the bovine-corneal-stroma group） at random. The defect was made, which was 7.5 mm in diameter and 1/3 of the thickness of the cornea, and the two kinds of materials were explanted to repair the defect. The vascularization and the changes of the operated eye were observed. The samples were taken at 2, 4 and 8 weeks for histological examination. Results The explanted materials were not melted or excluded. There were visible neovessels in both groups, yet there was no significant difference between them. According to the histological observation, there was severe inflammation in both groups 2 weeks after operation, the fibroblasts were proliferated, and the collagen fibers were disorganized; however, the reactions became milder from 4 weeks after operations, and the neovessles could be seen in groups A and B; at 8 weeks, the collagen fibers were more organized in groups A and B; however,there was still a small area of disorganized fibers left. Conclusion The two materials can lead to rejection to some extent, and so they need to be improved.
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2006年第4期463-466,共4页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家高技术研究发展计划(863)资助项目(2001AA216011)~~
关键词
羊膜
牛角膜基质
组织工程角膜
Amniotic membrane Bovine corneal stroma Tissue engineered cornea
