摘要
比较了金属螯合剂EDTA-Na2及各种蛋白酶水解分离人胃粘膜上皮细胞的方法.将胃粘膜组织置于不同分离液中,37℃振荡50min,结果显示:胶原蛋白酶、链霉蛋白酶、胰酶、EDTA-Na2单独使用及胰酶与EDTA-Na2联合使用,样本得到的细胞都较少;而胶原蛋白酶和链霉蛋白酶联合使用时,得到的细胞总数、上皮细胞比率及细胞活率都较高.用彗星电泳对胶原蛋白酶和链霉蛋白酶联合作用下的淋巴细胞进行了分析,结果表明,该条件不会对细胞的DNA造成损伤.
The methods of separating human gastric mucosa epithelia cells with EDTA-Na_2 and several proteinases were compared. Incubating the gastric mucosa in digestion solvent and shaking in 37℃. Treated by collagenase, pronase, trypsin, EDTA-Na_2 separately, and combination of EDTA-Na_2 and trypsin, the cell number released from every sample is low .However combination treatment by collagenase and pronase, the cell number, the proportion of epithelia cell or the cell viability are high. Determining the lymphocytes digested by the combination of collagenase and pronase with comet assay, the result suggest that the DNA of the cells are not damaged.
出处
《西北师范大学学报（自然科学版）》
CAS
2004年第2期59-61,94,共4页
Journal of Northwest Normal University(Natural Science)
基金
甘肃省自然科学基金资助项目(ZS991-A23-057-Y)
