摘要
目的 明确水通道蛋白-1(aquaporin-1,AQP-1)mRNA、抗凋亡基因Bcl-2 mRNA在鼻息肉组织中的表达、分布及其相关性,探讨AQP-1 mRNA在鼻息肉组织嗜酸粒细胞中表达的意义。方法 16例鼻息肉组织标本来源于鼻息肉切除的患者(女11例,男5例,年龄20-65岁);10例下鼻甲黏膜组织来源于有变应性鼻炎病史的鼻整形患者(女7例,男3例,平均年龄16-58岁)。上述标本取组织相邻切片,应用原位杂交方法检测AQP-1 mRNA和Bcl-2 mRNA的表达;以麦格-姬姆萨(May-Griunwald-Giemsa,MGG)染色法鉴别嗜酸粒细胞。结果 16例鼻息肉组织嗜酸粒细胞中均有AQP-1 mRNA和Bcl-2 mRNA表达,其阳性细胞表达率分别为(93.16±13.25)％;(84.74±12.10)％;而10例下鼻甲组织嗜酸粒细胞中均有Bcl-2 mRNA表达,但仅有4例表达AQP-1 mRNA;AQP-1mRNA和Bcl-2 mRNA在下鼻甲组织嗜酸粒细胞中阳性细胞表达率分别为(19.54±4.98)％和(16.45±3.12)％。AQP-1 mRNA与Bcl-2 mRNA在鼻息肉组织嗜酸粒细胞的表达呈明显正相关(r=0.875,P<0.01)。结论 AQP-1能够平衡鼻息肉组织嗜酸粒细胞内外液体渗透压,维持其存活,在嗜酸粒细胞抗凋亡中具有重要的意义。
Objective To study the significance of aquaporin-1 ( AQP-1 ) expression in the eosinophils of nasal polyps. The expression and location of AQP-1 mRNA and apoptosis associated gene Bcl-2 mRNA in nasal polyps were explored. Methods Sixteen nasal polyp samples were collected from 11 women and 5 men aged 20-65 years during routine endonasal surgery. Nasal mucosa specimens from the inferior turbinates of 10 patients with allergic rhinitis (7 women and 3 men, aged 16-58 years) , collected during septoplasty , were used as controls. The expression of AQP-1 mRNA and Bcl-2 mRNA was detected in serial adjacent sections by in situ hybridization and eosinophils were examined by stain MGG. Results AQP-1 mRNA expression was found in all 16 nasal polyps and in 4 of 10 inferior turbinate tissues, the mean expression rates were(93. 16 ± 13. 25) % and ( 19. 54 ± 4. 98 ) % , respectively. All 16 nasal polyps and 10 control nasal tissues expressed Bcl-2 mRNA, by the average rates of ( 84. 74 ± 12. 10 ) % and ( 16. 45 ± 3.12)%, respectively. The expression of AQP-1 mRNA was positively correlated with Bcl-2 mRNA expression in nasal polyps ( r = 0. 875 , P < 0. 01 ) . Conclusions AQP-1 contributes to the survival of eosinophils in nasal polyps by keeping the permeation balance of eosinophils.
出处
《中华耳鼻咽喉科杂志》
CAS
CSCD
北大核心
2004年第8期476-478,共3页
Chinese Journal of Otorhinolaryngology
