摘要
用化学致癌剂甲硝基亚硝胍（ＭＮＮＧ）对人类胃粘膜上皮细胞株ＧＥＳ－１进行不同方式处理，常规方法提取ＤＮＡ，经聚合酶链反应扩增Ｈ－ｒａｓ原癌基因后，以单链构象多态性检测Ｈ－ｒａｓ原癌基因，作者发现长期小剂量的ＭＮＮＧ打击ＧＥＳ－１可以诱导此细胞发生Ｈ－ｒａｓ基因改变，而大剂量短期处理的ＧＥＳ－１组未见Ｈ－ｒａｓ基因构型改变，提示化学致癌剂ＭＮＮＧ诱发Ｈ－ｒａｓ癌基因改变是一个缓慢、剂量叠加的过程，在ＭＮＮＧ诱发的胃癌中，Ｈ－ｒａｓ基因的改变不是首发事件。
A SV-40immortalized human gastric epithelial cell line GES-1 were
treatedwith carcinogen methyinironitrosoguanidine(MNNG)in high dosage(2×10-7mol/L)once
24hours twice;third time or low dosage(2×10-5mol/L)continually for 75 days.The H-rasgene of
these cells were amplified with PCR and the single stranded
conformationpolymorphism(SSCP)of the PCR product were analysed.The result has shown that
only onecell line MC-B which was exposed to MNNG at low dosage for 75 days gained a
differentband on page gel,compared to GES-1 data suggest that MNNG caused H-ras gene
pointmutation may not be a early event in the experimental system,instead it may be
aaccumlated effect on the gene.
出处
《内蒙古医学院学报》
1994年第2期107-109,共3页
Acta Academiae Medicinae Neimongol
关键词
化学致癌剂
胃粘膜
上皮细胞
癌基因
胃肿瘤
chemical carcinogen
methylnironitrosoguanidine
cell
line GES-1
polymerase chain reaction
single strand conformation polymorphism
