摘要
应用单抗双抗体夹心—ELISA和多抗双抗体夹心—ELISA检测旋毛虫病人血清循环抗原,其阳性率分别为67.7％(21/31)和72.2％(26/36),无显著件差异(P>0.05)。50例正常人两法皆为阴性。142例其它9种寄生虫病人中,两法除对20例囊虫病人中1例(5.0％)出现轻度交叉反应外均为阴性。对河南省新野县从事食品卫生人员调查,两法的阳性率分别为5.7％和5.9％,差异不显著。对两法循环抗原均呈阳性反应的14例进行了追踪观察,其中13例确诊为旋毛虫隐性感染。
Sera of 36 trichinosis patients were tested for circulating antigen with mono—and poly—clonal antibody—based indirect and direct double antibody sandwich—ELISA (McAb—IDAS and PcAb—DDAS)methods,and the positive rates obtained were 67.7% and 72.2o% respectively, while sera from 50 normal persons gave negative results. 142 sera from various parasitic infections(paragonimiasis, schistosomiasis japonica, clonorchiasis sinensis, hookworm disease, cysticercosis cellulosae, giardiasis, filariasis, echinococcosis and amebiasis) other than trichinosis showed a cross reation of 0.7%. A prevalence survey of trichinosis in workers employing oneself on food health made in Xinye County, Henan Province showed a positive rate of 5.7% by McAb—IDAS and 5.9% by PcAb—DDAS. These results indicated that circulating antigens detection is useful in the diagnosis of trichinosis, in the evaluation of drug efficacy as well as in the prevalence investigation of trichinosis.
出处
《中国寄生虫病防治杂志》
CSCD
1992年第2期102-105,共4页
Chinese Journal of Parasitic Disease Control
