摘要
应用旋毛虫幼虫溶解抗原,以快速-ELISA 法检测49例旋毛虫病人,全部皆为阳性;而100名正常人皆为阴性1105例其它9种寄生虫病人,仅日本血吸虫病人有交叉反应。并用此法对河南省新野县371例从事食品卫生工作人员和被宰杀的258头猪进行流行病学调查。证实该法具有较高的阳性符合率。对本试验的重复性、稳定和变异系数、存放时间、抗原经热处理等均进行了系统观察。用 Shewart控制图观察和控制试验结果。
The soluble antigen used was obtained from homogenates of T.spiralis larvea and fast-enzyme linked immunosorbent assay(Fast-ELISA)was adopted in this test for diagnos- ing trichinosis.Forty nine cases of trichinosis were examined and all of them showed positive reaction.Sera of 100 healthy persons gave negative results.All 71 sera from other parasitic diseases showed negative results except schistosomiasis with a relatively high cross reaction rate of 23.5%.Epicdemiologieal survey for trichinosis by fast-ELISA method was conducted in 371 employees working with pork handling and 258 slaughtered pigs naturally infected with T.spiralis in Xinye county,Henan province.The results of antibody examination were correlated with clinical and laboratory findings.The reproducbility was demonstrated to be good by analysing coefficient of variation.No significant changes occurred when antigen was kept in room temperature for 12 weeks or treated by heat(37℃ 30 min,56℃ 30 min,64℃ 30 min,100℃ 1,2,3 min,in water bath).The experimental results were analysed with con- trol graph of sheward.
关键词
旋毛线虫病
ELISA
流行病学
trichinosis
fast-ELISA
epidemiological investigation
