摘要
研究了影响大豆幼胚培养体细胞胚胎发生频率的9个因素。诱导体细胞胚胎发生的适宜幼胚长度为4mm;随着供俸植株发育阶段的提高诱导频率下降;最适基本培养基为 MS 培养基;蔗糖浓度从1.5%提高到9%,诱导频率逐渐下降;过高的维生素 B_1浓度对胚胎发生不利;2,4-D 的诱导效果优于 NAA,适宜的2,4-D 浓度为20ppm;光、暗处理与生长素种类和浓度之间存在交互作用;接种方式对诱导频率影响很大,体细胞胚只在下表皮与培养基接触的幼子叶的上表皮上产生,当上表皮与培养基接触时,两个表皮都不能产生体细胞胚;被试的所有基因型都能被诱导胚胎发生,不同基因型的诱导频率存在差异。
Nine factors which influence the frequency of somatic embryo-genesis from immature embryos of soybean were studied, The best length ofimmatural embryo for somatic embryogenesis was 4 mm, Embryogenesis fre-quency decreased as the developing stage of the donor plant increased,Thebest medium was MS,The efficiency of embryogenesis decreased as the sucro-se concentration increased from 1.5 to 9%.Overhigh concentration of vitaminB_1 was not good for the somatic embryogenesis.The initiation efficiency of2,4-D was superior to that of N.A.A,the best concentration of 2,4-D was20 ppm.There was on interaction between light-dark,treatment and the typeand concentration of auxin,To a great extent,the embryogenesis was influ-enced by ways in which the immatural cotyledons contacted the medium,Somatic embryos produced only on the adaxial surface of the cotyledons which contacted the medium.However,when the adaxial surface contactedthe medium,both surfaces couldn't produce somatic embryos.Genotypicvariation in the frequency of embryogenesis was noted.However,embryo-genesis was induced from all tested genotypes。
出处
《植物学通报》
CSCD
1992年第2期38-43,共6页
Chinese Bulletin of Botany
关键词
大豆
组织培养
体细胞胚胎发生
Soybean(Glycine max)
Tissue culture
Somatic Embryogenesis
