摘要
大豆原生质体培养诱导再生植株一直为国内外学者所关注。如Kao(1970)和Miller等(1971)从悬浮培养的大豆细胞分离出原生质体,经培养获得了愈伤组织。但在以后的多年中进展不够大。据报道,已从幼荚子叶、幼苗根、叶肉组织和悬浮培养细胞等外植体游离出原生质体,经培养获得愈伤组织,并进行了大量的分化研究,但最终都未能得到再生植株。
Protoplasts were isolated from immature cotyledons of Glycine max L. cultivars Sidou No. 11 and Tiefeng No. 8 and cultured in Gellan Gum-solidified beads modified MS medium (Murashige and Skoog, 1962) with 0, 1-0. 2mg/1 2, 4-D, 0. 5-1. 0mg/1 BA, 40 mg/1 as-paragine,40 mg/1 glutamine. Plating, efficiencies of 57-65% were obtained at 10 days. Upon regular dilution microcalli of 1-2 mm in size were obtained in 50-60 days , which were transferred to MSB medium (MS salts; Murashige & Skoog, 1962 +B3 organics; Gamborg et al. , 1968)with 0. 5mg/1 2,4-D,0. 5mg/1 BA, 1% sucrose for further prollfe ration. After subculture on MSB medium containing 5. 0 mg/1 NAA ,0. 5 mg/1 KT, 0. 5 mg/1BA,3% sucrose ,em-bryoids were formed. Embryoids were matured on MSB medium with 0. 5 mg/1 NAA,0. 5mg/1KT,1% suerose.then developed into plantlets. Plantlets were transferred into 1/2MBS mediumwith 6. 1-0. 5mg/1 GA3, 1 % sucrose for further growth. The regeneration frequency of Sidou No. 11 and Teifeng No. 8 were 16% and 2%.
出处
《大豆科学》
CAS
CSCD
北大核心
1993年第3期249-251,共3页
Soybean Science
