摘要
目的 :探讨芦笋提取液对人恶性黑色素瘤A3 75细胞增殖的抑制作用以及对其凋亡的诱导作用。方法 :用四甲基噻唑氮蓝比色法测定A3 75细胞活力 ;流式细胞术 (FCM)检测A3 75细胞的凋亡诱导及杀伤作用 (P <0 .0 1 )。结果 :芦笋提取液与人恶性黑色素瘤细胞株A3 75细胞生长抑制率之间有明显的浓度依赖关系 ,浓度分别为 2 0 0mg/ml、4 0 0mg/ml、6 0 0mg/ml和80 0mg/ml时 ,A3 75细胞的凋亡率及坏死率分别为 2 .39%、8.85 %、8.71 %、5 .1 6 %和 1 .6 5 %、1 .5 1 %、2 2 .1 0 %、82 .80 %。结论 :芦笋提取液能显著抑制恶性黑色素瘤A3 75细胞的增殖 ,且诱导A3 75细胞总的凋亡率和坏死率与其浓度之间存在着依赖关系。
Objective:To explore the effects of asparagus extract on the inhibition of proliferation and apoptosis in human melanoma A 375 cells.Methods:The cell proliferation inhibition was measured by methyl thiazolyl tetrazolium(MTT) colorimetric assay;apoptosis and necrosis in A 375 cells were analysed by flow cytometry.Results:There was obviously a concentration dependent relationship between the asparagus extract and the inhibition of A 375 cells proliferation( P <0.01).The rates of apoptosis and necrosis in A 375 cells were 2.39%, 8.85 %,8.71%,5.16% and 1.65%,1.51%,22.10%,82.80% respectively as treated at doses of asparagus extract 200 mg/ml,400 mg/ml,600 mg/ml and 800 mg/ml.Conclusions:Asparagus extract can obviously inhibit the proliferation of A 375 cells.There exists a concentration dependent relationship between asparagus extract and the total rates of apoptosis and necrosis.
出处
《蚌埠医学院学报》
CAS
2004年第2期95-97,共3页
Journal of Bengbu Medical College
基金
安徽省教育厅自然科学研究资助项目 (No .2 0 0 1kJ171)
