摘要
用75%乙醇为溶媒,超声提取芦笋下脚料,获得提取物,对提取物中总皂苷、总多糖、总游离氨基酸含量进行分析;用体外细胞培养法观察该提取物对正常及ConA诱导的小鼠脾淋巴细胞增殖的影响;以整体口服给药实验观察该提取物对正常小鼠免疫功能的影响,剂量分别为25、100、400mg/(kg.d),连续给药7d。结果表明:该提取物总皂苷含量高达18.20%、总多糖及总游离氨基酸含量分别为14.86%和12.63%;体外培养对脾淋巴细胞增殖无影响,但对ConA诱导的脾淋巴细胞增殖呈低剂量促进高剂量抑制的作用;口服给药能增强小鼠碳廓清能力和ConA诱导的小鼠脾淋巴细胞转化增殖作用。芦笋下脚料乙醇提取物具有较明确的增强免疫功能的作用。
In this study, 75% ethanol extract from asparagus scraps obtained by ultrasonic-assisted extraction was analyzed for the contents of total saponins, total polysaccharides and free amino acids. Meanwhile, its in vitro effect on the normal and Con A-induced proliferation of mouse spleen lymphocytes was studied, and the effect of oral administration at doses of 25, 100 mg/kg and 400 mg/kg on immune functions in normal mice was also observed. The ethanol extract contained 18.20% total saponins, 14.86% total polysaccharides and 12.63% free amino acids. It had no influence on normal spleen lymphocytes in vitro, whereas the ConA-induced proliferation of spleen lymphocytes could be promoted at 25 mg/kg but suppressed at 400 mg/kg by it. Oral administration of this extract enhanced carbon clearance capacity in mice and the ConA- induced proliferation of spleen lymphocytes. Therefore, this asparagus extract has remarkable immunoenhancing functions.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第1期277-280,共4页
Food Science
基金
国家公益性行业(农业)科研专项(201003074-5)
关键词
芦笋提取物
皂苷
多糖
游离氨基酸
免疫功能
asparagus extract
saponin
polysaccharide
free amino acid
immune function
