摘要
目的 :探讨氧化苦参碱 (OM )对肝癌细胞诱导血管内皮细胞 (VEC)增殖的抑制作用。方法 :用MTT法检测不同浓度OM对VEC、人肝癌SMMC 772 1细胞增殖及SMMC 772 1细胞诱导VEC增殖的影响。结果 :OM浓度为 2 .5～ 1 0mg mL时 ,对VEC增殖的抑制率为 4 .6 %～ 36 .4 % ;OM浓度为0 31 3～ 1 0mg mL时 ,对肝癌细胞增殖的抑制率为 2 5 3%～ 88 6 1 % ;经浓度为 1 2 5～ 1 0mg mLOM作用后的肝癌细胞培养液对VEC增殖的抑制率为 7 6 9%～ 38 4 6 % ;OM浓度为 0 6 2 5～ 1 0mg mL时 ,对肝癌细胞培养液诱导的VEC增殖的抑制率为 3 5 7%～ 5 7 5 4%。结论
Objective To investigate inhibitory effect of Oxymatrine(OM) on vascular endothelial cell (VEC) proliferation induced by hepatocellular carcinoma cell.Methods Inhibitory effect of OM on VEC proliferation induced by human hepatocellular carcinoma cell was detected with the MTT method. Results When the concentration of OM was 2.5-10 mg/mL, the inhibitory rate of OM to VEC proliferation was 4 6%-36 4%.When the concentration was in the range of 0 313-10 mg/mL,its inhibitory rate to SMMC 7721 cell proliferation was 2 53%-88 61%.The inhibitory rate of the cultured medium of SMMC 7721 cell acted with OM 1 25-10 mg/mL to VEC proliferation was 7 69%-38 46%.When oxymatrine was 0 625-10 mg/mL,the inhibitory rate to VEC proliferaton induced by cultured medium of SMMC 7721 cell was 3 75%-57 14%. Conclusion OM can inhibit VEC proliferation induced by hepatocellular carcinoma cell.
出处
《肿瘤防治杂志》
2003年第7期707-709,共3页
China Journal of Cancer Prevention and Treatment
