摘要
在pH3.0～6.5的条件下,镧(Ⅲ) 曲利本红(TR)与硫酸新霉素(NEO)或硫酸庆大霉素(GEN)反应生成三元红色离子缔合物,其最大吸收波长位于388nm,摩尔吸收光系数(ε)为4.86×104、2.10×104L·mol-1·cm-1;最大负吸收波长位于432nm(NEO)和428nm(GEN),摩尔吸收光系数(ε)为1.38×104、1.19×104L·mol-1·cm-1。当用双波长叠加时,ε值为6.24×104(NEO)、3.28×104(GEN)L·mol-1·cm-1。探讨了适宜的反应条件和主要分析化学性质。该法用于市售药物中新霉素、庆大霉素含量的测定,结果满意。
In the medium of pH 3.0～6.5, La(Ⅲ) and trypan red(TR) can react neomycin sulfate(NEO) or gentamycin sulfate(GEN) to form three ingredient red ion association complexes . The maximum absorption wavelengths are at 388 nm. Their molar absorptivities (ε) are 4.86×10^(4)(NEO) and 2.10×10^(4)(GEN) L·mol^(-1)·cm^(-1), respectively. The maximum negative absorption wavelengths are located at 432 nm(NEO) and 428 nm(GEN). Their molar absorptivities(ε) are 1.38×10^(4)(NEO) and 1.19×10^(4)(GEN) L·mol^(-1)·cm^(-1), respectively. When the dual-wavelengths overlapping absorption spectrometry is used to determine the concentrations of aminoglycoside antibiotics, their molar absorptivities ε are (6.24)×10^(4)(NEO) and 3.28×10^(4)(GEN) L·mol^(-1)·cm^(-1), respectively. The optimum reaction conditions and the chief properties of analytical chemistry are studied. The method has been applied to the determination of the content of the commercial neomycin and gentamycin antibiotics with satisfactory results.
出处
《分析科学学报》
CAS
CSCD
北大核心
2004年第1期51-53,共3页
Journal of Analytical Science
