摘要
秋水仙碱在硫酸溶液中水解后产生弱的稳定的荧光。在ｐＨ４～ｐＨ６范围内，镧（Ⅲ）离子可与水解秋水仙碱发生络合反应，络合后荧光强度增加了６倍。最大激发及发射波长为２４６ｎｍ和４３０ｎｍ。在０．１～１０ｍｇ／Ｌ浓度范围内，荧光强度与浓度有良好的线性关系。本方法已用于片剂中秋水仙碱含量的测定，测定下限为０．１ｍｇ／Ｌ，相对标准偏差为４．３％。
table fluorescence is emitted after colchicine is hydrolyzed in hot sulfuric
acid solution. The fluorescence intensity is increased by 6 times after the hydrolyzate is
complexed with lanthanum() ion in the pH range of 46, upon which a fluorescence method has
been developed for the determination of colchicine in tablet. There is a good linear relationship
between the fluorescence intensity and the concentration of colchicine in the range of 0.1-10
mg/L.The excitation and emission peaks were at 246nm and 430nm, respectively.
出处
《分析试验室》
CAS
CSCD
1999年第3期74-76,共3页
Chinese Journal of Analysis Laboratory
