摘要
目的 研究血管紧张素 - 1型受体 (AT1 R)基因 A116 6 / C多态性和血管紧张素转换酶 (ACE)基因I/ D多态性与冠状动脉 (冠脉 )粥样硬化病变严重程度的关系。方法 采用聚合酶链反应 -限制性片段长度多态性(PCR- RFL P)技术检测冠心病 (CHD)组 (130例 )和正常对照组 (90例 ) ACE和 AT1 R基因多态性。对 CHD患者进行冠脉造影 ,判定冠脉病变支数 (狭窄程度≥ 75 % )和 Jeopardy危险记分。结果 CHD组 ACE- DD基因型频率(38.5 % )显著高于对照组 (14 .4 % ) ,P <0 .0 1。 AT1 R- AC基因型在两组间差异无显著性 (13.1%和 10 % ;P >0 .0 5 )。冠脉病变支数和危险记分在 ACE三种基因型间差异无显著性 (P>0 .0 5 ) ,但在 AT1 R- AC基因型患者显著高于 AT1 R- AA基因型患者 (P <0 .0 5 )。结论 AC- EDD基因型是 CHD发病的独立危险因素之一 ,但与冠脉病变严重程度不相关。 AT1 R-
Objective To explore the relation of angiotensin-converting enzyme(ACE) and angiotensin Ⅱ type 1 receptor(AT 1R) gene polymorphism to coronary heart disease(CHD) and the severity of coronary artery stenosis.Methods The insertion(I)/deletion(D) of ACE gene polymorphism and AT 1R gene polymorphism(an A→C transversion at nucleotide position 1166) were detected by the means of polymerase chain reaction and restriction fragment length polymorphism(PCR -RFLP) in 130 CHD patients and 90 normal controls.130 CHD patients who underwent coronary angiography were determined the number of affected coronary vessels(≥75% stenosis) and coronary Jeopardy score.Results DD genotype of ACE was more frequent in CHD patients than that in the control group (38.5%vs14.4%,P<0.01).The frequency of the AT 1R A/C genotypes did not differ between the patients and the controls (13.1%vs10%,P>0.05).Neither the number of affected coronary vessels nor the coronary score differed among the ACE I/D genotypes (P>0.05),but they were significantly higher in patients with AT 1R -AC genotype than in those with AA genotype (P<0.05).Conclusion DD genotype is a risk factor for CHD and myocardial infarction(MI) in Chinese people,and not be involved in the development of the coronary artery stenosis.The AT 1R -C allele is involved in the development of the coronary artery stenosis.
出处
《山东医药》
CAS
北大核心
2004年第4期10-12,共3页
Shandong Medical Journal
