摘要
目的 :研究冠心病患者血管紧张素Ⅱ 1型受体 (AT1 R)基因A1 1 6 6 /C多态性分布特点及与冠脉病变严重程度的关系。方法 :对 1 30例冠心病患者进行冠状动脉 (冠脉 )造影 ,判定冠脉病变支数和危险记分。采用聚合酶链反应 限制性片段长度多态性 (PCR RFLP)技术检测冠心病组和健康对照组 (90例 )AT1 R基因多态性。结果 :检测出AA和AC型 2种基因型 ,冠心病组基因型及等位基因频率与对照组比较差异无统计学意义 (P >0 .0 5 ) ,但AC基因型患者冠脉病变支数和冠脉危险记分显著高于AA基因型患者 (P <0 .0 5 )。结论 :AT1 R基因A1 1 6 6 /C多态性与冠心病的发生无关 。
Aim:To explore the association of angiotensin Ⅱ type 1 receptor(AT 1R) gene polymorphism with coronary heart disease and the severity of coronary artery stenosis. Methods: The total of 130 CHD patients with coronary angiography were determined the number of affected coronary vessels(>75% stenosis) and coronary Jeopardy score. The AT 1R gene polymorphism(an A→C transversion at nucleotide position 1166) was detected by the means of polymerase chain reaction(PCR) and restriction fragment length polymorphism(RFLP) in CHD patients and 90 controls. Results: Two genotypes, AA and AC, were detected. The frequency of the AT 1R A/C genotype had no significant difference compared with the controls. However, the number of affected coronary vessels and the coronary Jeopardy score were significantly greater in patients with the AT 1R AC genotype than those of the AA genotype( P <0.05). Conclusion: There is no association between AT 1R gene A1166/C polymorphism and the occurrence of coronary heart disease in Chinese people from Central China area, but the AT 1R C allele is involved in the development of the coronary artery stenosis.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2004年第4期584-586,共3页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省重大科技攻关基金资助项目 9712 0 0 10 0
