摘要
目的:建立泮托拉唑对映体拆分和对映体光学纯度测定方法。方法:用α1-酸糖蛋白手性固定相,考察了缓冲液的种类和pH、有机改性剂的种类和比例、流速及柱温对泮托拉唑对映体拆分的影响。采用Chiral-AGP柱(150 mm×4.6 mm,5μm,配有手性AGP预柱),以10 mmol·L-1醋酸铵缓冲液(pH 5.5)-乙腈(93:7)为流动相,在流速为0.9 mL·min-1,检测波长为290 nm,柱温为20℃的条件下拆分泮托拉唑对映体。结果:用α1-酸糖蛋白手性固定相能完全分离泮托拉唑对映体,分离度达1.77,并测定了泮托拉唑对映体过量百分率。结论:流动相pH和有机改性剂含量是影响对映体分离的重要因素,可以以调节流速和柱温来提高柱效、改善分离。
Objective:To develop a chiral separation method for the enantiomers of pantoprazole. Method:Influences of the pH value of the mobile phase, the organic modifiers, the flow rate and the column temperature on the enantiomeric separation of pantoprazole using α1 - acid glycoprotein chiral stationary phase were investigated. Pantoprazole enantiomers were separated on a Chiral -AGP column (150 mm ×4. 6 mm, 5μm)with a mobile phase consisted of 10 mmol· L-1 NH4OAc ( pH 5. 5 ) - acetonitrile (93: 7) at a flow rate of 0. 9 mL· min-1. Results: Pantoprazole enantiomers were separated on an α1 - acid glycoprotein chiral stationary phase and the resolution was 1. 77. The values of enantiomeric excess of pantoprazole were determined under optimized conditions. Conclusion: The pH value of the mobile phase, the organic modifer concentration, the flow rate and the temperature of column were very important when optimizing the enantiomeric separation of pantoprazole.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2004年第1期1-4,共4页
Chinese Journal of Pharmaceutical Analysis
基金
国家自然科学基金资助项目(项目批准号:39930180)
关键词
α1—酸糖蛋白
泮托拉唑
对映体
手性固定相
chiral stationary phase, enantiomeric separation, pantoprazole, enantiomeric excess
