摘要
应用HPLC方法在 10 0mm× 4.6mm的Chiral AGP手性固定相柱上 ,分离氟布洛芬、西替利嗪、罗哌卡因等手性药物和反式 2 苯基环丙基硼酸的对映体混合物 .考察了流动相pH值和缓冲液浓度等对样品在柱上的保留和分离的影响 ,研究了流动相中加入四种不带电荷的有机添加剂乙腈、甲醇、正丙醇和异丙醇以及加入不同浓度的有机添加剂等对对映体分离性的影响 .此方法用于手性药物合成中的对映过量值的测定 ,取得满意的效果 .
The separation of the enantiomers of flurbiprofen, ropivacaine hydrochloride, cetirizine and trans-2-phenylcyclopropylboronic acid using HPLC on a 100 mm x 4.6 mm Chiral-AGP has been accomplished. The effect of pH value and concentration of mobile phase buffer on the chromatographic performance of enantiomers of racemates was examined. The influence of properties and concentration of organic solvents (acetonitrile, methanol, n-propyl alcohol and isopropyl alcohol) in mobile phase on retention time and enantioselective separation level of racemates was studied. This method had been successfully applied to the determination of the optical purity of the drug enantiomers resolved from their racemates.
出处
《有机化学》
SCIE
CAS
CSCD
北大核心
2002年第12期1018-1021,共4页
Chinese Journal of Organic Chemistry
