摘要
以微晶纤维素和苯甲酰氯为原料合成了纤维素三苯甲酸酯,涂敷于氨丙基硅烷化硅胶上制成手性固定相。并以β 环糊精衍生物为手性流动相添加剂在手性柱上进行对映体的拆分。结果表明:当使用手性固定相和含有手性添加剂的流动相分离手性化合物时存在手性两相协同作用,其中以2,3,6 三甲基 β 环糊精(TM β CD)为流动相添加剂时的效果最好。
Cellulose tribenzoate (CTB) was synthesized. Elemental analysis and IR spectra indicated that most of the hydroxy groups of the microcrystallin cellulose were converted into ester groups. The silanized gel was coated with CTB by using tetrahy drofuran as solvent, which was used as the swtationary phase of high performance liquid chromatography. When β-cyclodextrin(β-CD), dimethyl β-cyclodextrin (DM-β-CD) and trimethyl β-cyclodextrin (TM-β-CD) were used as mobile phase additives, the separation of enantiomers of phenylalanine and tryptophyan showed two-phase synergistic effects.
出处
《分析试验室》
CAS
CSCD
北大核心
2004年第10期13-15,共3页
Chinese Journal of Analysis Laboratory
基金
国家自然科学基金资助项目(30160092)
关键词
Β-环糊精衍生物
纤维素三苯甲酸酯
高效液相色谱
流动相添加剂
手性两相协同作用
High performamce liquid chromatography
Cellulose tribenzoate
β-cyclodextrin derivative
Mobile phase additive
Two-phase synergistic effect
