摘要
从堆肥中筛选得到两株分解纤维素的菌株 ,一株为高温单孢菌Q 0 ,另一株为芽孢杆菌Q 3,对Q 0、Q 3及这两株菌组成的混合菌产纤维素酶条件进行了研究。结果表明 ,混合菌分解棉花和滤纸纤维素的分解率均比单一菌株高 ,其分解率分别为 6 9%和 6 2 %。混合菌产酶最适温度为 5 0℃ ,pH 7.5。在以棉花纤维为惟一碳源时 ,混合菌产生的纤维素酶可达 10 1个酶活单位 ,比菌Q 0高近 4 0个酶活单位。Q 0、Q 3和混合菌利用有机氮源优于无机氮源。
Two strains with considerable capability of cellulosed gradation were selected from compost. One strain is Thermomonospora sp. Q-0 and the other is Bacillus sp. Q-3. We investigated the conditions for the formation of cellulase of the Q-0?Q-3 and the mixed strains, which contains both of the strains. The result show mixed strains can degrade cotton and filter paper more effectively than Q-0 and Q-3, the degradation rates were 69% and 62% respectively. The suitable temperature for cellulase is 50 ℃ and pH is 7.5. Cotton can induce the cellulase formation, the cellulase activity of mixed strains was 100 IU/ml, almost 40 IU/ ml higher than Q-0. Inaddition,Q-0?Q-3 and mixed culture take the priority on organic nitrogen sources to inorganic nitrogen sources.
出处
《天然产物研究与开发》
CAS
CSCD
2003年第6期510-512,共3页
Natural Product Research and Development
