摘要
以新华滤纸为唯一碳源 ,从垃圾堆肥中筛选能够分解纤维素的菌株共 39株 ,采用刚果红鉴别培养基进行识别 ,获取透明圈较大的菌株 10株。在此基础上 ,进行液体培养 ,测定酶活 ,得到 1株酶活较高的曲霉B - 6 (Aspergillussp)。将B - 6与绿色木霉 (Trichodermasp)AS3 3711进行了参比试验 ,以对筛选工作进行评定。经过固体、液体发酵对比试验 ,发现B - 6与AS3 3711有相近的产酶性能。B - 6在固、液发酵中酶活分别达到 39 2IU、14 9IU ,而AS3 3711则分别为 16 6IU与 15 7IU。且B - 6较AS3 3711有更强的液化CMC的能力 ,B - 6在 2 4h内即能使 3%CMC完全液化 ,而AS3 3711则需 96h。
The 39 strains of cellulose-decomposing microorganisms were isolated from rubbish compost by Dubos liquid medium with Xinhua filter paper as the sole carbon source.We got 10 colonies surrounded by some distinct zones of clearing in cellulose-congo red agar medium from the 39 strains above.By measuring the enzyme activity of liquid culture filtrates,we got a strain(termed B-6)with higher cellulase activity.In order to evaluate our screening,the results of liquid and solid fermentation were compared between strain B-6 and AS33 711.We found out that they had similar enzyme-producing property.The enzyme activity of B-6 reached 39.2 and 14.9units,comparied to AS3.3711 with 16.6 and 15.7 units in solid and liquid fermentation respectively.In addition,B-6 had higher capacity of liquefying CMC.It could liquidize 3% CMC completely within 24 hours,while AS3.3711 needed 96h to do it.
出处
《生物技术》
CAS
CSCD
2001年第2期27-30,共4页
Biotechnology
关键词
筛选
纤维素降解菌
纤维素酶
分离
screening
cellulose-decomposing microorganisms
cellulase
