摘要
利用限制性培养技术,从沼液中筛选出1组木质纤维素降解复合菌系。采用木质纤维素失重法,对复合菌系的培养条件进行研究。单因素试验结果表明,稻秆经碱液浸泡后降解率最高,最佳氮源为"蛋白胨+酵母浸粉+硝酸铵"的复合氮源。采用五因素二次通用旋转组合设计进行培养条件的优化组合试验及分析,建立了碳源添加量、氮源添加量、pH、温度、接种量与秸秆降解率间的数学模型,确定最佳的碳、氮源添加量和培养条件为:稻秆3.0g·L-1、蛋白胨4.0g·L-1、酵母浸粉0.8g·L-1、硝酸铵1.2g·L-1,初始pH7.5～8.0,接种量7%(体积比),培养温度30℃,在此条件下,稻秆的降解率可达到64.98%。
A microbial community with capacity of degrading lignocellulose effectively was enriched from biogas slurry by the method of limited cultivation. The effect of cultural conditions on the capability of lignocellulose degradation was investigated with gravimetry of rice straw. The results of single factor design indicated that the rice straw degradation ratio was the highest under the condition of pretreatment with sodium hydroxide among all pretreatments. The percentage of straw degradation was the highest by using peptone, yeast powder and ammonium nitrate as multiple nitrogen source. The five factors design of universal quadratic rotation combination was used to optimize cultural conditions. A mathematical model of the relation among carbon source, nitrogen source, pH, temperature, quantity of inoculation and percentage of straw degradation was established. The optimum cultural conditions were straw 3.0 g·L^-1 of carbon source, peptone 4.0 g·L^-1 plus yeast powder 0.8 g·L^-1 plus ammonium nitrate 1.2 g·L^-1of nitrogen source, the pH value 7.5 to 8.0, 7%（v/v）of inoculation quantity and at 30 ℃, respectively. The percentage of straw degradation was up to 64.98% under above conditions.
出处
《黑龙江八一农垦大学学报》
2008年第6期62-67,共6页
journal of heilongjiang bayi agricultural university
基金
国家科技支撑计划(2006BAD04A09)
(2006BAD07A10)
(2008BADC4B01)
国家863计划(2007AA100705)
黑龙江省科技攻关计划项目(GA07B501)
(GB06B502)
(JC01B509)
黑龙江省普通高等学校新世纪优秀人才培养计划(1153-NCET-003)
黑龙江省普通高等学校青年学术骨干支持计划项目(1151G030)
关键词
复合菌系
木质纤维素分解
培养条件
二次通用旋转组合设计
microbial community
lignocellulose degradation
cultural conditions
universal quadratic rotation combination
