摘要
目的探讨人参皂苷Rg1对体外软骨细增殖及表达的影响。方法在成功分离培养人软骨细胞后,以MTT法检测软骨细胞活性并绘制其生长曲线图,以不同浓度Rg1作用于IL-1α诱导的软骨细胞,检测培养液中SOD的活性及Ⅱ型胶原含量。结果浓度为40μmol/L的Rg1在3～5d内使细胞增殖率升高;浓度为40μmol/L的Rg1可对抗IL-1α,降低SOD及降解Ⅱ型胶原的含量。结论人参皂苷Rg1可促进体外软骨细增殖及其表型的表达。
Objective To study the effect of ginsenoside Rg1 on chondrocytic proliferation and cell phenotype.Methods The activity of chondrocyte was detected by MTT method and its growth curve was drawn out after culture of chondrocyte.Ginsenoside affects the induced chondrocyte by IL- 1α in different density.The activity of superoxide dismutase (SOD) and the content of collagen Ⅱ in the culture fluid were detected after IL- 1α - induced chondrocytes were cultured with ginsenoside Rg1 at different concentrations.Results Ginsenoside Rg1 in 40 μ mol/L could accelerate the proliferation of chondrocytes within 3～ 5 days, and counteract the decrease of SOD activity and the degeneration of collagen Ⅱ induced by IL- 1α .Conclusion Ginsenoside Rg1 can promote the proliferation of chondrocytes and its expression of cell phenotype.
出处
《中药新药与临床药理》
CAS
CSCD
2004年第1期4-6,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广东省中医药管理局资助项目(编号102038)。
