摘要
目的 :探讨人参皂甙Rg1对MPP+诱导SHSY5Y细胞凋亡的保护作用及其可能的机制。方法 :用吖啶橙溴化乙锭染色观察SHSY5Y细胞凋亡率 ,流式细胞仪检测线粒体跨膜电位及细胞内活性氧 (ROS)水平 ,WesternBlot法检测bcl 2、bax蛋白表达水平。结果 :经 10 μmol·L- 1Rg1预处理后 ,MPP+诱导的SHSY5Y细胞凋亡受到明显的抑制 ,虽然线粒体跨膜电位无明显改变 ,但细胞内ROS下降 ,而bcl 2蛋白表达水平增加 ,bax蛋白表达水平减少。结论 :Rg1可抑制MPP+诱导的SHSY5Y细胞凋亡 ,其作用机制可能是通过清除ROS、调节bcl 2、bax蛋白表达水平起作用。
AIM: To explore the possible anti apoptotic mechanism of ginsenoside Rg1 on MPP + induced cellular apoptosis. METHODS: The apoptosis of SHSY5Y induced by MPP + was observed by AO EB staining. Flow cytometry was used to quantitate mitochondrial transmembrane potential (△ψm) and reactive oxygen species (ROS) . Western Blot was used to detect the expression of bcl 2 and bax proteins in SHSY5Y cells. RESULTS: MPP + induced apoptosis in SHSY5Y cells was obviously inhibited by pretreatment with 10 μmol·L -1 Rg1. Although there was no difference of mitochondrial transmembrane potential between Rg1 pretreated groups and MPP + groups, the level of ROS in Rg1 pretreated groups decreased, the expression of bcl 2 protein increased and expression of bax protein decreased. CONCLUSION: Rg1 protects against MPP + induced apoptosis in SHSY5Y cells and the effect may be attributed to its remove of ROS and its regulation of expression of bcl 2 and bax proteins.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2002年第5期412-416,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
福建省自然科学基金资助项目 (№C0 110 0 15 )
