摘要
研究人参皂甙Ｒｇ１对神经细胞凋亡的抑制作用。用原代培养的大鼠脑皮层神经细胞为实验模型。结果表明，Ｒｇ１能增强细胞活性，降低ＬＤＨ的释放，减轻细胞核形态的改变，减少ＤＮＡ断裂，增加细胞膜流动性，抑制细胞凋亡。提示细胞凋亡与细胞膜流动性关系密切；Ｒｇ１的抗衰老作用与抗凋亡作用有关。
Ginsenoside Rg1 is an active principle isolated from Panax ginseng CA Meyer. Primary neuronal culture was performed with different concentrations of Rg1. On the 14th day, the culture was changed to serum free medium. On the 16th day, neurons were harvested and assayed under microscope and fluorescence microscope. The result showed that serum withdrawal from the medium induced apoptosis in primary cultured cortical neurons. Rg1 (1, 10 μmol·L -1 ) was shown to inhibit apoptosis and protect neurons against injury. Membrane fluidity was measured using fluorescence spectrophotometer in five groups of cultured cortical neurons: control (complete medium), apoptosis (serum free medium), Rg1 0 1 μmol·L -1 , Rg1 1 μmol·L -1 and Rg1 10 μmol·L -1 . Neurons were isolated enzymatically and loaded with the fluorescent dye, DPH (1,6 diphenyl 1,3,5 hexatriene). Membrane fluidity in control neurons (η 1 2928±0 1426) was shown to be significantly higher than that in apoptosis cells (η 2 9277±0 2004). The membrane fluidity of neurons treated with Rg1 (0 1, 1, 10 μmol·L -1 ) increased significantly (η 2 9172±0 1604, 2 4731±0 2187, 1 6436±0 1483). These findings indicate a substantial alteration of membrane fluidity with neuronal apoptosis. Increment of membrane fluidity provides an aspect of elucidating the mechanism of Rg1′s anti apoptosis function.
出处
《药学学报》
CAS
CSCD
北大核心
1997年第6期406-410,共5页
Acta Pharmaceutica Sinica
基金
国家自然科学基金
关键词
人参皂甙
RG1
神经细胞
细胞凋亡
膜流动性
Ginsenoside Rg1
Cortical neurons
Apoptosis(programmed cell death)
Membrane fluidity
