摘要
用喹烯酮 14 C标记法测定猪血液中喹烯酮的浓度并对喹烯酮静脉单次给药后在猪体内的药代动力学进行了分析。猪 6头 ,单剂量0 .4 0 6 5 mg·kg-1(比活度 2 4 .6 μci· mg-1)静注给药 ,于不同的时间采集血样。消化液(HCl O4:H2 O2 =1:1.2 5 )消化完全一定量血样后 ,以液体闪烁谱仪计数法进行含量测定。喹烯酮线性范围为 0 .0 10～ 3.170 μg· g-1(r=0 .9980 ) ,最低检测量为 1.1ng,最低检测浓度为 0 .0 11μg· g-1,平均方法回收率 (10 2 .75±5 .2 6 ) % ,精密度考察喹烯酮日内、日间相对标准偏差小于 6 %。喹烯酮以原药的形式代谢排泄 ,静注给药符合二室开放模型 :t1/ 2α=0 .1899h,t1/ 2β=4 .5 5 2 8h,Kel=0 .86 5 4h-1,AUC=0 .0 0 92 5 mg· h-1· L-1。结果表明 :喹烯酮静注给药后 ,其在体内分布快而广 ,消除相对也较快。本方法专一性强 ,快速 ,准确。
A method of label by 14 C is described for the analysis and Pharmacokinetics Studies of quinocetone in pig blood. Normal pigs were given with quinocetone by iv at dose of 0.4065 mg·kg -1 .The concentrations of quinocetone were determined by an liquid flamed chromatographic machine methed developed in our laboratory. The ranges of pyroline calibration curves were 0.010～3.170 μg·g -1 (r=0.9980), Detection Limits were 1.1 ng and the Lowest detectable concentration of pyroline was 0.011 μg·g -1 , while the average recovery of the method was (102.75±5.26) %. The RSD of intra day and inter day assays all less than 6%. blood drug Concentration time Courses of quinocetone after iv administration was found to be fitted to a two Compartment open model and its pharmacokinetic parameters was as follows: T1/2α =0.1899 h,T 1/2β =4.5528 h,Kel=0.8654 h -1 ,AUC=0.00925 mg·h -1 ·L -1 .Results show:quinocetone was distributed rapidly and wide,eliminated fast.The method is quick, precise. Its pharmacokinetic parameters are base for its use in clinic
出处
《动物医学进展》
CSCD
2003年第3期78-79,共2页
Progress In Veterinary Medicine
基金
中国乡镇企业的横向合作项目
