摘要
本文以含 35 0mg/kg和 4 0 0mg/kg喹乙醇的饲料经 99d分别连续投喂鲤和银鲫 ,以测定喹乙醇在鲤和银鲫体内的蓄积及其对鲤、银鲫的影响。试验结果表明 ,无论是银鲫单养还是鲤鲫混养 ,无论是鲤还是银鲫 ,试验组喹乙醇在肝脏组织中蓄积量最高 ,99d单养银鲫组可达 10 0 5 7± 0 0 15 (3)mg/kg ,鲤鲫混养组银鲫、鲤分别达 10 10 7± 0 2 2 6 (3)mg/kg,9 883± 0 0 32 (3)mg/kg ,肾脏次之 ,99d单养鲫鱼组的银鲫 ,鲤鲫混养组的银鲫、鲤分别为 7 4 94± 0 0 6 4 (3)mg/kg,7 777± 0 138(3)mg/kg和 7 6 0 8± 0 0 86 (3)mg/kg ,肌肉中较低 ,分别为 0 1170± 0 0 0 3(3)mg/kg ,0 16 0± 0 0 0 3(3)mg/kg,0 4 86± 0 0 0 6 (3)mg/kg;经检验三种组织器官中的蓄积量差异显著 (P <0 0 5 )。由于喹乙醇在鱼体内的蓄积 ,虽鲤鲫混养组的银鲫、鲤的相对增重率达到 10 2 7%和 110 7% ,但是它们的抗应激反应率明显下降 ,而且随喹乙醇摄入量的增加下降趋势更为明显 ,当给予一定程度的应激刺激后 ,单养鲫鱼组的银鲫应激反应率由 5 8d的4 4 8%上升到 99d的 6 4 3% ,鲤鲫混养组的银鲫和鲤分别由 5 8d的 6 6 7%和 4 6 2 %分别上升到 99d的 88 3%和76 9% ,无论是 5 8d还是 99d ,试验组与对照组间差异显著
In order to determine the accumulation of olaquindox in common carps (Cyprinus carp) and prussian carp (Carassius auratus gibelio) ,these two kinds of fishes were fed with diets which contented the olaquindox at the doses of 350mg/kg and 400mg/kg respectively,and the method for the determination of olaquindox residues in flesh for export (SN0197 94,An industry standard for export in the People's Republic of China)and high performance liquid chromatography(HPLC)was used.The experiments were conducted for 99 days. Culture styles included monoculture of prussian carp,polyculture of prussian carp and common carp.The results showed that,of the three selected organs(liver,kidney and muscle),the accumulation of olaquindox in the liver was the highest,followed by the kidney and the muscle.On the 99th day,olaquindox's concentration in the livers was 10 057± 0 015(3)mg/kg in prussian carp of monocultural groups,which was 10 107±0 226(3)mg/kg in prussian carp and was 9.883± 0 032(3)mg/kg in common carps of polycultural group. Those in the kidneys respectively were:7 494±0 064(3)mg/kg, 7 777±0 138(3)mg/kg, 7 608±0 086(3)mg/kg and 0 1170±0 003(3)mg/kg, 0 160±0 003(3)mg/kg, 0 486±0 006(3)mg/kg in the muscles. Although relative growth rate in the polycultural carps were 102.7% and 110 7% respectively,their anti stress ability was reduced apparently with the increasing intake of olaquindox.After the stimulation were administered,the stress rate of prussian carp in monoculture climbed from 44.8% on 58d to 64.3% on 99d,that of prussian carp and common carps in polyculture climbed from 66.7% and 46.2% on 58d to 88.3% and 76.9% on 99d.No matter on 58d or on 99d,there are significantly different between the control and test groups (P <0 05).It's observed that the number of the inflammatory cells grew,the volume of the cell shrank,and the cell nuclear lessened.It was found that extensively granular and vacuolar degeneration and necrosis of liver cells in every test groups.Renal tubule cells degenerate and capillary dilate.The net crossing membrane of spleen festers and the structure of spleen were destroyed seriously.The distinction between each two cells became obscure.Histopathology indicated the following changes:tissues of disease fishes were hemorrhage,their cells appeared edema and necrosis.Which caused extensive tissue damage,blood vessels were enlarged and seriously damaged.A large number of erythrocytes in the blood vessels and tissues were deformed,broke and haemolyzed.The study showed that olaquindox is toxic to cultural fishes and must be forbidden in aquaculture.
出处
《水生生物学报》
CAS
CSCD
北大核心
2005年第1期13-19,共7页
Acta Hydrobiologica Sinica
基金
农业部重点科研项目 (渔 95 B 0 0 0 1)资助
