摘要
本实验是以黄色短杆菌T_(6—13)的诱变株L—亮氨酸产生菌D—R—4为出发菌株,经青霉素、甘氨酸、溶菌酶作用制备原生质体,形成率达91.30％,再生率达53.68％;然后对原生质体进行紫外线、利福平、氯化锂复合诱变处理;在再生培养基平皿上培养,获得再生突变株,从中挑取单独菌落,进行摇瓶发酵筛选,已选育出一株57—4S号高产稳定菌株;经氨基酸分析仪测定其发酵液L—亮氨酸产量由出发菌株的17.35mg/ml提高到23.45mg/ml提高了35％。发酵液中主要副酸——异亮氨酸含量很少。
A starting strain, L-leucine producer D-R-4(derived from B.flavum T6-13) was treated with penicillin, glycine and lysozyme. The formation and the regeneration rate of protoplasts were 91.3% and 53.0% respectively. The protoplasts were then multimutated with UV rifamapin and Licl and single colonies of regenerated strains were obtained in regeneration medium plates. The strains were investigated for their abilities of L-leucine production and strain 57-4s giving 23.45 mg/ml of L-leucine was slected as a high-yield producer, the increase being 35% over the yield by its parent strain (17.35mg/ml) . The level of the main by-product--isoleucine was very low.
出处
《微生物学杂志》
CAS
CSCD
1992年第1期11-16,共6页
Journal of Microbiology
