摘要
人肺癌A549细胞产生的免疫抑制因子(TDSF)作用于巨噬细胞(Mφ)5h,即可抑制白细胞介素-1(IL-1)的产生;如持续作用24 h、48h则抑制作用更强。与对照组相比P<0.05,<0.01.当TDSF存在时,经尼龙毛处理的胸腺细胞对外源性IL-1反应显著减弱,表明TDSF能抑制IL-1作用。ConA刺激未经尼龙毛处理的胸腺细胞可产生增殖,但TDSF使其增殖抑制.表明:TDSF能抑制IL-1的产生及作用,并能干扰由IL-1介导的其他反应.TDSF可通过抑制胸腺细胞增殖从而阻止新鲜T细胞的补充。
Abstract A549,a human lung cancer cell line, produces a tumorderived immu-nosuppressive factor (TDSF), which inhibited the production of IL-1 after its action on macrophages for 5 h The inhibition was much stronger if TDSF acted on macrophage culture for 24, 48 h. The response of thymocytes treated with nylon wool to exogeneous IL-1 was significantly suppressed in the presence of TDSF, suggesting that TDSF can inhibit the action of IL-1. The thymocytes untreated with nylon wool could proliferate after being stimulated with Con A. The proliferation was significantly suppressed by TDSF in dose- dependent manner. Thise findings suggest that the inhibitory action of TDSF on T cell activation and proliferation is associated with IL-1, and that TDSF may exert an inhibitory effect on other reactions mediated by IL-1. Furthermore, TDSF can reduce the supplement of new T cells by inhibiting the proliferation of thymocytes.
出处
《同济医科大学学报》
CSCD
北大核心
1992年第1期9-12,共4页
Acta Universitatis Medicinae Tongji
基金
国家自然科学基金
关键词
肺癌细胞
免疫抑制因子
白细胞介素
lung cancer cell
immunosuppressive factor
interleukin l
thymocyte
