摘要
目的 研究低剂量的亚硒酸钠和氯化镉联合作用对离体大鼠肝细胞DNA损伤的影响。方法 在 2 185、4 3 75和 8 75 0 μmol L的剂量条件下 ,亚硒酸钠和氯化镉联合作用 ,用单细胞凝胶电泳检测大鼠肝细胞DNA损伤。 结果 2 185 μmol L的亚硒酸钠对 2 185 μmol L的氯化镉引起的DNA损伤拮抗作用不明显 ,但有拮抗作用的趋势 ;4 3 75μmol L亚硒酸钠对 4 3 75 μmol L氯化镉引起的DNA损伤有明显的拮抗作用 ;而在 8 75 0 μmol L的剂量下 ,亚硒酸钠与氯化镉存在相互拮抗的关系。结论 在硒和镉对大鼠肝细胞DNA损伤的联合作用中 ,较低剂量的亚硒酸钠对氯化镉没有拮抗作用 ,而在一定的剂量条件下 。
Objective This study was conducted to explore effects of selenium and cadmium acted jointly on rat hepatocellular DNA damage at relative low doses in vitro.Methods At the dose of 2^185,4^375 and 8^750 μmol/L,sodium selenite acted jointly with cadmium chloride.Rat hepatocellular DNA damage was measured with single cell gel electrophoresis.Results Sodium selenite at dose of 2^185 μmol/L could not inhibit DNA damage caused by cadmium chloride at the dose of 2^185 μmol/L in rat liver cells significantly.Sodium selenite at the dose of 4.375 μmol/L had antagonistic effect on DNA damage induced by cadmium chloride at the same dose obviously.At the dose of 8^750 μmol/L,sodium selenite and cadmium chloride could antagonize each other significantly.Conclusion Although selenium could not inhibit DNA damage induced by cadmium at relative low doses,selenium and cadmium could antagonize each other at certain doses on DNA damage in rat liver cells.
出处
《卫生毒理学杂志》
CAS
CSCD
北大核心
2003年第4期200-202,共3页
Journal of Health Toxicology
基金
国家自然科学基金资助 (30 2 71 1 1 0
39670 62 6)
