摘要
目的:探讨急性心肌梗死(Acute Myocardial Infarction,AMI)患者循环内皮细胞(CEC)核DNA改变的特点及动态监测copeptin含量变化的临床意义。方法:应用单细胞凝胶电泳技术(SCGE)检测60例AMI急性期(12h)患者和38例健康对照CEC核DNA的损伤;采用双抗体夹心免疫分析法测定血浆和肽素水平。结果:AMI组患者(93.33±5.75)%的CEC核DNA电泳呈明显的"彗星"状,其彗尾DNA含量为(41.12±3.77)%;对照组(10.53±3.54)%的CEC核DNA表现为"彗星"状,其彗尾DNA%含量为(7.33±2.53)%。AMI组与对照组彗星率、彗尾DNA含量相比均差异有统计学意义。(均P<0.001)。AMI组患者血浆copeptin水平显著升高,2dcopeptin含量显著升高,与对照组相比差异有统计学意义(P<0.01);12h、7dcopeptin含量明显升高,与对照组相比差异有统计学意义(P<0.05);12hcopeptin含量与7dcopeptin含量相比差异无统计学意义(P>0.05)。结论:AMI急性期患者CEC核DNA有明显损伤,动态监测copeptin水平,有利于AMI患者的治疗与预后。
Objective： To investigate changes in nuclear DNA of circulating endothelial cells and dynamic monitoring value of copeptin in patients with acute myocardial infarction（AMI）.Methods：The damage of nuclear DNA of circulating endothelial cells was assayed by single cell gel electrophoresis（SCGE）and agarose gel electrophoresis（AGE） in 60 acute-phase patients with AMI,and 38 health adults were controlled.copeptin levels in plasma were detected by sandwich immunoluminometric assay.Results：The nuclear DNA of AMI group was obviously demonstrated like comet and majority of nuclear DNA were demonstrated like circle in control group.AMI group showed a higher percentage of comet cells than the control group（93.33%±5.75% and 10.53%±3.54%,respectively,P0.001）,and their tail DNA content was significantly increased in AMI（41.12%±3.77% and 7.33%±2.53%,respectively,P0.001）.2 d Opeptin level in AMI patients was significantly higher than that of the control group（P0.01）;12 h、7 d copeptin level in AMI patients was significantly higher than control group（P0.05）;copeptin level in AMI patients was no significant difference between 12 h and 2 d（P0.05）.Conclusions： The damage of nuclear DNA for circulating endothelial cell was significant in AMI,dynamic monitor of copeptin level may contribute to the treatment or prognosis of AMI.
出处
《海南医学院学报》
CAS
2012年第4期459-462,共4页
Journal of Hainan Medical University
基金
海南省自然科学基金项目(808234)~~
关键词
心肌梗死
循环内皮细胞
单细胞凝胶电泳
核DNA损伤
和肽素
Acute myocardial infarction
Circulating endothelial cell
Single cell gel electrophoresis
Nuclear DNA damage
Copeptin
