摘要
〔目的〕研究肝硬化患者红细胞CD58数量表达的变化与其病情严重程度的相关性。〔方法〕将戊二醛固定的2 %红细胞悬液定量“液相包被”到V型板中 ,依次加入鼠抗人CD58单克隆抗体、碱性磷酸酶标记的羊抗鼠第二抗体 ,洗板后加底物显色 ,移显色液于比色板中 ,测定其A40 5吸光值。本文对 113例肝硬化病人的红细胞CD58进行测定 ,同时采用AU60 0全自动生化仪检测其胆碱酯酶 (CHE)、胆红素 ,采用ACL2 0 0检测凝血酶原活动度 (PTA)。〔结果〕肝硬化患者红细胞CD58数量表达都显著低于正常对照 ,并且其变化与病情严重程度积分比较差异高度显著 (t =-4 12 ,P =3 96×10 -5) ,与PTA的变化关系密切 (t =2 13 ,P =0 0 1)。〔结论〕红细胞CD58数量变化与肝硬化病情严重程度密切相关 ,该试验可作为综合判断肝硬化病情变化的重要指标。
objectiveTo study the relationship between the quantitative express of CD 58 on erythrocyte and the changes of CHE,PTA in patients with hepatacirrhosis.Investigate it's significance in clinic.〔Methods〕Glutaraldehyde-fixed red blood cells(RBC)are analyzed in V well microtitre plates.To the cell buttons in each well,mouse monoclonal anti-human CD 58was added of the first antibodies.The microtitre was incubated at 37℃ for 45min,followed by three washes in 1% S/BSA.The second antibody of anti-mouse IgG conjugated to alkaline phosphatase was then added,and was incubated in the same condition.Substrate was added and incubated at 37℃ for 90min.The supernatant was transferred to a clean U microtitre plate to facilitate reading in a plate reader at 405nm(A405).〔Results〕The results showed that the A405 of CD 58 on erythrocytes in the diseases with cirrhosis is all significantly lower than that of control groups.There is a good relationship between the decreassed number of CD 58 on erythrocytes and the changes of PTA in hepatacirrhosis and the servity of the diseases(t=-4.12,P=3.96×10 -5).〔Conclusion〕The results suggest that there is a good relationused as an important parameter for clinic diagnosis in patients with hapatacirrhosis.
出处
《中国卫生检验杂志》
CAS
2003年第6期715-716,共2页
Chinese Journal of Health Laboratory Technology
